Volume 40 Issue 2
Feb.  2023
Turn off MathJax
Article Contents
TIAN Zhenhua, HE Jingxuan, WANG Ying. Development and analysis of a novel collagen-sodium humate composite hydrogel[J]. Acta Materiae Compositae Sinica, 2023, 40(2): 1050-1059. doi: 10.13801/j.cnki.fhclxb.20220321.005
Citation: TIAN Zhenhua, HE Jingxuan, WANG Ying. Development and analysis of a novel collagen-sodium humate composite hydrogel[J]. Acta Materiae Compositae Sinica, 2023, 40(2): 1050-1059. doi: 10.13801/j.cnki.fhclxb.20220321.005

Development and analysis of a novel collagen-sodium humate composite hydrogel

doi: 10.13801/j.cnki.fhclxb.20220321.005
Funds:  Natural Science Foundation of Shaanxi Province (2019JQ-027); National Natural Science Foundation of China (21706151); Scientific Research Program Funded by Shaanxi Provincial Education Department (21JK0549)
  • Received Date: 2022-01-14
  • Accepted Date: 2022-03-12
  • Rev Recd Date: 2022-02-23
  • Available Online: 2022-03-22
  • Publish Date: 2023-02-01
  • Hydrogel was one of main materials for medical dressings due to the excellent performance such as high elasticity, high water content, cold effect, strong moisture retentiveness and variable shape. Collagen (COL) had good biocompatibility and can promote cell proliferation. And Sodium humate (NaHA) possessed hemostatic, anti-inflammatory and other biological functions. Therefore, a novel collagen-sodium humate composite hydrogel was prepared by mixing collagen and NaHA at various COL∶NaHA ratios and collagen self-assembly, which was expected to be applied as medical dressing. Then the interaction between collagen and NaHA and the microstructures and properties of composite hydrogels were investigated. The collagen triple helix is not affected, although hydrogen bonds and electrostatic interaction occur between collagen and NaHA. At COL∶NaHA≤4∶6, the hydrogels possess good compatibility due to the shielding effect of NaCl on electrostatic binding; however, the coagulation takes place with the further increase in NaHA content. At COL∶NaHA=4∶6, the percentage of NaHA incorporated into collagen fibrils is highest and 93.2%, and the compatibility between collagen and NaHA is good; therefore, the properties of composite hydrogel consisting of mature fibrils with D-periodicity are optimum. Moreover, about 80% of NaHA still remain in hydrogel, indicating its release is slow. The thermal stability improves by 34.9°C, and storge modulus and loss modulus are 31.89 Pa and 3.99 Pa, respectively. Furthermore, the pore sizes of the lyophilized composite hydrogels decrease and the pores become dense and well-distributed. The composite films have significant rises in hydrophilicity.


  • loading
  • [1]
    LI S, WANG L, ZHENG W, et al. Rapid fabrication of self-healing, conductive, and injectable gel as dressings for healing wounds in stretchable parts of the body[J]. Advanced Functional Materials,2020,30(31):2002370. doi: 10.1002/adfm.202002370
    MOHAMADI S, NOROOZNEZHAD A H, MOSTAFAEI S, et al. A randomized controlled trial of effectiveness of platelet-rich plasma gel and regular dressing on wound healing time in pilonidal sinus surgery: Role of different affecting factors[J]. Biomedical Journal,2019,42(6):403-410. doi: 10.1016/j.bj.2019.05.002
    STEVENSON F J. Humus chemistry: Genesis, composition, reactions[M]. 2nd ed. New York: John Wiley & Sons, 1994.
    顾刚果, 耿宝琴, 雍定国. 黄腐植酸钠的抗凝作用[J]. 现代应用药学, 1988, 4(5):8, 41.

    GU Gangguo, GENG Baoqin, YONG Dingguo. Anticoagulant effect of sodium xanthate[J]. Application of Modern Medicine,1988,4(5):8, 41(in Chinese).
    JI Y, ZHANG A, CHEN X, et al. Sodium humate accelerates cutaneous wound healing by activating TGF-beta/smads signaling pathway in rats[J]. Acta Pharmaceutica Sinica B,2016,6(2):132-140. doi: 10.1016/j.apsb.2016.01.009
    张爱军, 顾慧莹, 闫志勇, 等. 不同基质和pH值的腐植酸钠凝胶剂对大鼠皮肤创伤愈合的影响[J]. 中国药房, 2013, 21(24):1933-1935.

    ZHANG Aijun, GU Huiying, YAN Zhiyong, et al. Effect of sodium humic gel with different matrix and pH on wound healing of rats[J]. China Drug Store,2013,21(24):1933-1935(in Chinese).
    顾其胜, 王帅帅, 王庆生, 等. 海藻酸盐敷料应用现状与研究进展[J]. 中国修复重建外科杂志, 2014, 28(2):255-258. doi: 10.7507/1002-1892.20140055

    GU Qisheng, WANG Shuaishuai, WANG Qingsheng, et al. Application status and research progress of alginate dressing[J]. Chinese Journal of Reparative and Reconstructive Surgery,2014,28(2):255-258(in Chinese). doi: 10.7507/1002-1892.20140055
    JIN J, JI Z, XU M, et al. Microspheres of carboxymethyl chitosan, sodium alginate, and collagen as a hemostatic agent in vivo[J]. ACS Biomaterials Science & Engineering,2018,4(7):2541-2551.
    LIU X, ZHENG M, WANG X, et al. Biofabrication and characterization of collagens with different hierarchical architectures[J]. ACS Biomaterials Science & Engineering,2020,6(1):739-748.
    SUN L, LI B, SONG W, et al. Comprehensive assessment of nile tilapia skin collagen sponges as hemostatic dressings[J]. Materials Science and Engineering: C,2020,109:110532. doi: 10.1016/j.msec.2019.110532
    刘晨阳, 马建中, 张跃宏. 胶原蛋白基纳米复合材料的性能及界面研究进展[J]. 复合材料学报, 2021, 38(6):1691-1702.

    LIU Chenyang, MA Jianzhong, ZHANG Yuehong. Progress on properties and interface of collagen-based nanocomposites[J]. Acta Materiae Compositae Sinica,2021,38(6):1691-1702(in Chinese).
    MORGENSTERN L, MICHEL S L, AUSTIN E. Control of hepatic bleeding with microfibrillar collagen[J]. Archives of Surgery,1977,112:941-943. doi: 10.1001/archsurg.1977.01370080039005
    DOILLON C J, WHYNE C F, BRANDWEIN S, et al. Collagen-based wound dressings: Control of the pore structure and morphology[J]. Journal of Biomedical Materials Research,1986,20:1219-1228. doi: 10.1002/jbm.820200811
    DOILLON C J, SILVER F H. Collagen-based wound dressing: Effects of hyaluronic acid and firponectin on wound healing[J]. Biomaterials,1986,7(1):3-8. doi: 10.1016/0142-9612(86)90080-3
    BHASKAR K, MOHAN C K, LINGAM M, et al. Development of SLN and NLC enriched hydrogels for transdermal deli-very of nitrendipine: In vitro and in vivo characteristics[J]. Drug Development and Industrial Pharmacy,2009,35(1):98-113. doi: 10.1080/03639040802192822
    TEZGEL Ö, DISTASIO N, LAGHEZZA-MASCI V, et al. Collagen scaffold-mediated delivery of NLC/siRNA as wound healing materials[J]. Journal of Drug Delivery Science and Technology,2020,55:101421. doi: 10.1016/j.jddst.2019.101421
    LAGHEZZA M V, TADDEI A R, COURANT T, et al. Characterization of collagen/lipid nanoparticle-curcumin cryostructurates for wound healing applications[J]. Macromolecular Bioscience,2019,19(5):e1800446. doi: 10.1002/mabi.201800446
    RIEDE U N, JONAS I, KIRN B, et al. Collagen stabilization induced by natural humic substances[J]. Archives of Orthopaedic and Trauma Surgery,1992,111:259-264. doi: 10.1007/BF00571520
    GERT J K, ROBERT E M H. Experiments on collagen-humic interactions speed of humic uptake, and effects of diverse chemical treatments[J]. Journal of Archaeological Science,1995,22:263-270. doi: 10.1006/jasc.1995.0028
    LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature,1970,277:680-685.
    PIETRUCHA K. Changes in denaturation and rheological properties of collagen-hyaluronic acid scaffolds as a result of temperature dependencies[J]. International Journal of Biological Macromolecules,2005,36(5):299-304. doi: 10.1016/j.ijbiomac.2005.07.004
    DING C, ZHANG M, LI G. Preparation and characterization of collagen/hydroxypropyl methylcellulose (HPMC) blend film[J]. Carbohydrate Polymers,2015,119:194-201. doi: 10.1016/j.carbpol.2014.11.057
    Bio-Rad Laboratories Inc, Informatics Division. Sadtler spectral handbooks[M]. Berkeley: Bio-Rad Laboratories Inc, 2004: 116.
    冯文坡, 祁元明, 汤克勇. 胶原-羟基磷灰石/阿拉伯树胶复合材料的制备与表征[J]. 复合材料学报, 2010, 27(6):113-119.

    FENG Wenpo, QI Yuanming, TANG Keyong. Preparation and characterization of collagen-hydroxyapatite/gum Arabic composite[J]. Acta Materiae Compositae Sinica,2010,27(6):113-119(in Chinese).
    易菊珍, 梁子倩, 张黎明. 腐植酸钠/聚丙烯酰胺水凝胶吸水性能的研究[J]. 中山大学学报(自然科学版), 2007, 46(2):36-40. doi: 10.3321/j.issn:0529-6579.2007.02.010

    YI Juzhen, LIANG Ziqian, ZHANG Liming. Studies on sodium humate/polyacrylamide hydrogels(I) synthesis and water absorption properties[J]. Journal of Sun Yat-sen University (Medical Sciences),2007,46(2):36-40(in Chinese). doi: 10.3321/j.issn:0529-6579.2007.02.010
    丁翠翠. 胶原/HPMC共混体系中大分子相互作用及相态转变特性研究[D]. 成都: 四川大学, 2015.

    DING Cuicui. Macromolecular interaction and phase transition in collagen/HPMC blends[D]. Chengdu: Sichuan University, 2015(in Chinese).
    LI Y P. The mechanism of collagen self-assembly: Hydrophobic and electrostatic interactions[D]. Gainesville: The University of Florida, 2009.
    PIELESZ A. Temperature-dependent FTIR spectra of collagen and protective effect of partially hydrolysed fucoidan[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2014,118:287-293. doi: 10.1016/j.saa.2013.08.056
    田振华, 何静瑄, 王颖, 等. 基于二维红外技术研究氧化羧甲基纤维素钠/胶原的相互作用及热稳定性[J]. 光谱学与光谱分析, 2021, 9(41):2782-2788.

    TIAN Zhenhua, HE Jingxuan, WANG Ying, et al. Interaction and thermal stability of oxidized carboxymethyl cellulose/collagen based on two-dimensional infrared spectroscopy[J]. Spectroscopy and Spectral Analysis,2021,9(41):2782-2788(in Chinese).
    TIAN H L, LI C H, LIU W T, et al. The influence of chondroitin 4-sulfate on the reconstitution of collagen fibrils in vitro[J]. Colloids and Surfaces B: Biointerfaces,2013,105:259-266. doi: 10.1016/j.colsurfb.2013.01.005
    DING C, ZHANG M, TIAN H, et al. Effect of hydroxypropyl methylcellulose on collagen fibril formation in vitro[J]. International Journal of Biological Macromolecules,2013,52:319-326. doi: 10.1016/j.ijbiomac.2012.10.003
    LEE H J, AHN S H, KIM G H. Three-dimensional collagen/alginate hybrid scaffolds functionalized with a drug delivery system (DDS) for bone tissue regeneration[J]. Chemistry of Materials,2011,24(5):881-891.
    PARK J H, LEE G S, SHIN U S, et al. Self-hardening microspheres of calcium phosphate cement with collagen for drug delivery and tissue engineering in bone repair[J]. Journal of the American Ceramic Society,2011,94(2):351-354. doi: 10.1111/j.1551-2916.2010.04314.x
    ARAFAT M T, TRONCI G, WOOD D J, et al. In-situ crosslinked wet spun collagen triple helices with nanoscale-regulated ciprofloxacin release capability[J]. Materials Letters,2019,255:126550. doi: 10.1016/j.matlet.2019.126550
    FRANCIS-SEDLAK M E, URIEL S, LARSON J C, et al. Characterization of type I collagen gels modified by glycation[J]. Biomaterials,2009,30(9):1851-1856. doi: 10.1016/j.biomaterials.2008.12.014
    WU K J, WANG C Y, LU H K. Effect of glutaraldehyde on the humoral immunogenicity and structure of porcine dermal collagen membranes[J]. Archives of Oral Biology,2004,49(4):305-311. doi: 10.1016/j.archoralbio.2003.10.002
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(11)  / Tables(1)

    Article Metrics

    Article views (373) PDF downloads(34) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint