Evaluation method of biomaterial degradation based on <i>in vivo</i> imaging system

LIU Xiuyun; CHEN Danli; GUAN Qifeng; LIU Tian; YANG Fenghe; NIU Xufeng

doi:10.13801/j.cnki.fhclxb.20201215.009

Volume 38 Issue 2

Feb. 2021

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LIU Xiuyun, CHEN Danli, GUAN Qifeng, et al. Evaluation method of biomaterial degradation based on in vivo imaging system[J]. Acta Materiae Compositae Sinica, 2021, 38(2): 487-495. doi: 10.13801/j.cnki.fhclxb.20201215.009

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LIU Xiuyun, CHEN Danli, GUAN Qifeng, et al. Evaluation method of biomaterial degradation based on in vivo imaging system[J]. Acta Materiae Compositae Sinica, 2021, 38(2): 487-495. doi: 10.13801/j.cnki.fhclxb.20201215.009

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Evaluation method of biomaterial degradation based on in vivo imaging system

doi: 10.13801/j.cnki.fhclxb.20201215.009

LIU Xiuyun^{1, 2
,},
CHEN Danli^{1, 2
,},
GUAN Qifeng^{1, 2
,},
LIU Tian^{1, 2
,},
YANG Fenghe^{1, 2
,},
NIU Xufeng^{1, 2
,
,}

1.
School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China
2.
Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100083, China

Received Date: 2020-04-23
Accepted Date: 2020-06-02

Available Online: 2020-12-16

Publish Date: 2021-02-15

Abstract

Abstract

Degradable material is an important part of biomaterials, its degradation performance in vivo has great influence on the final success after implantation. Thus, the method of evaluating the degradation of the materials in vivo is crucial to the evaluation of material performance. The traditional method to estimate the degradation in vivo needs to take out different batches of degradation samples at each sampling point, which prevents the continuous measurement of the degradation process and it needs a large number of samples. In vivo imaging system (IVIS) has the characteristics of non-invasive and strong operability, which provides a way to solve the problems above. In this study, we established a method to detect the degradation performance of degradable materials with IVIS. The research method was that the near-infrared fluorescent dye was labeled on the degradable materials by chemical reaction, and then using the change of fluorescence intensity to reflect the degradation degree of the materials. The in vivo degradation experimental results show that the fluorescent labeling materials prepared by this method have high fluorescence stability, and the fitting effect of fluorescence intensity and mass loss in the process of material degradation is good (R² = 0.9994). In conclusion, this method solves the problem of large amount of samples in the traditional method of measuring material degradation, and improves the continuity of the experimental process.
- degradable medical polymer materials,
- in vivo degradation,
- in vivo imaging system,
- fluorescence labeling,
- evaluation method
Long Abstrsct
Innovation Points

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References(25)

References

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