An experimental study of temperature-dependent mechanical behavior of acrylonitrile butadiene styrene (ABS) was performed at a range of temperatures (243 K, 263 K, 296 K, 333 K, 353 K, 383 K) under uniaxial tension condition. In this temperature range, ABS was in the glass state and glass transition region. The results show that the yield stress and ultimate tensile stress decrease linearly but Young's modulus decreases nonlinearly with the increase of temperature. The material parameters of the model at different temperature were obtained by using a sim-flow optimization tool. The temperature-dependent two-layer viscoplasticity constitutive model was established. This constitutive model can predict the stress-strain curve of ABS well at different temperatures between 243 K and 383 K.