纳米羟基磷灰石/聚酰胺复合材料屏障膜的体外生物相容性
详细信息In vitro biocompatibility of nano-hydroxyapatite/ polyamide composite barrier membranes
-
摘要: 用相转移法制备了用于引导骨再生的纳米磷灰石/聚酰胺66(n-HA/PA66)和载银纳米羟基磷灰石/二氧化钛/聚酰胺66(Ag-HA-TiO2/PA66)复合生物材料屏障膜, 并通过与骨髓基质干细胞(BMSC)共培养评价了其体外生物相容性。扫描电子显微镜(SEM)观察显示，制备的复合材料膜为不对称多孔膜，一面是孔径小于10μm的微孔层，另一面是孔径在30～200μm的大孔结构。四唑盐比色(MTT)和流式细胞术(FCM)试验结果表明：n-HA/PA66膜具有良好的细胞亲和力，有利于BMSC的黏附、生长和减少凋亡；Ag-HA-TiO2/PA66 膜也具有良好的生物相容性，但加速了BMSC的凋亡。2种膜的结构和生物相容性能够满足引导骨组织再生膜材料的要求。
-
关键词:
- 屏障膜 /
- 引导骨再生 /
- 纳米羟基磷灰石/聚酰胺 /
- 生物相容性 /
- 流式细胞术
Abstract: Nano-hydroxyapatite/polyamide 66(n-2HA/PA66) and Ag loaded hydroxyapatite/titania/polyamide 66 (Ag-HA-TiO2/PA66) composite membranes for guided bone regeneration (GBR) were prepared by the phase inversion method. Biocompatibility of the two kinds membranes was assessed via 32(4，52dimethylthiazol222yl)22，52 diphenyl-tetrazolium-bromide assay (MTT) and flow cytometry (FCM) test. The fabricated membranes have asymmetric porous structure. SEM analysis reveals that pores less than 10μm and pores with a size ranging from 30μm to 200μm distribute in the micropore layer and the spongy structure layer，respectively. MTT and FCM results indicate that the two kinds membranes have good cell affinity，while Ag-HA-TiO2/PA66 membrane can promote cell apopotosis to some extent. The structure and biocompatibility of the fabricated membranes can meet the requirements of GBR. -
-
计量
- 文章访问数: 1836
- HTML全文浏览量: 69
- PDF下载量: 169
下载:
