Preparation and properties of gelatin@Poly (L-lactic acid) core-shell nanofibers
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摘要:
通过静电纺丝技术制备的纤维膜具有高比表面积、高孔隙率等优点，在生物医学、环境、能源等领域被广泛应用。但由于传统的静电纺丝的局限性，即混合体系均一，仅能纺出实心单一组分的纤维，使得纳米纤维在临床应用中受到了限制。同轴静电纺丝技术是基于传统静电纺丝技术改进，采用同轴喷头，将两种及以上的高分子材料制备成不同形态结构的纤维。一方面，可以将不具可纺性的材料包覆在核内，提高高分子材料的性能。另一方面，核内载药，可以保护药物防止失活。其次还能借助其特殊结构，控制药物释放。本文通过同轴静电纺丝技术成功制备了核壳结构的GEL@PLLA纳米纤维膜，探究了纺丝工艺参数对纤维形貌的影响，评价了核壳结构纳米纤维膜细胞相容性，为该膜在骨组织工程领域的应用提供理论依据，并为新型骨生物材料的研发提供新思路。 GEL@PLLA 核壳纤维激光共聚焦图GEL@PLLA Core shell fiber laser confocal image BMSC在纳米纤维膜上的形态Morphology of BMSC on nanofiber membrane (a) Nanofiber membrane × 1000； (b) Nanofiber membrane × 3000； (c) Nanofiber membrane × 10000； (a)纳米纤维膜×1000；(b)纳米纤维膜×3000；(c)纳米纤维膜×10000； Abstract: Coaxial electrospinning technology is based on the improvement of traditional electrospinning technology. The fiber material prepared by it not only has a high specific surface area, but also has a special core-shell structure, which can encapsulate active molecules, maintain their biological activity, and achieve the goal of sustained release. This article uses coaxial electrospinning technology to prepare gelatin (GEL)@poly (L-lactic acid) (PLLA) nanofiber membranes with core-shell structure. The morphology, structure, and properties of nanofiber membranes were characterized using scanning electron microscopy (SEM), laser confocal microscopy (LSCM), tensile testing, and contact angle testing. The influence of electrospinning process parameters on the morphology of nanofibers was explored, and the biocompatibility of nanofiber membranes was investigated. The results indicate that the prepared GEL@PLLA The surface of core-shell nanofibers is smooth and has a clear core-shell structure. Increasing the flow velocity ratio between the core-shell layers increases the average diameter of the nanofibers from 231.41 nm to 279.49 nm; Increasing the spinning voltage gradually reduces the fiber diameter; By increasing the receiving distance, the fiber diameter decreases first and then increases; Core-shell structure GEL@PLLA The contact angle of the fiber membrane is 126.7°, which exhibits hydrophobicity compared to pure GEL fiber membrane; The mechanical test results show that GEL@PLLA Core shell nanofibers have good flexibility and elasticity; The results of in vitro cell culture showed that BMSC can GEL@PLLA The adhesion and proliferation on the core-shell fiber membrane indicate that the core-shell nanofiber membrane has good biocompatibility. This study can lay the foundation for the further application of fiber membranes in drug-controlled release systems and biomedical fields.-
Key words:
- Gelatin /
- Poly(L-lactic acid) /
- Coaxial electrospinning /
- Core shell structure /
- Nanofiber /
- drug-controlled release
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图 1 不同核壳溶液推进速度比下GEL@PLLA核壳纤维的SEM图
Figure 1. Under Different Propulsion Velocity Ratios of Core Shell Solutions GEL@PLLA SEM images of core-shell fibers
图 2 不同电压下GEL@PLLA核壳纤维的SEM图
Figure 2. Under different voltages GEL@PLLA SEM images of core-shell fibers
图 3 不同接收距离下GEL@PLLA核壳纤维的SEM图
Figure 3. Under different reception distances GEL@PLLA SEM images of core-shell fibers
图 5 GEL@PLLA 核壳纤维傅里叶红外光谱图
Figure 5. GEL@PLLA Fourier transform infrared spectroscopy of core-shell fibers
图 6 GEL@PLLA纳米纤维膜的应力-应变曲线
Figure 6. Stress-strain curve of GEL@PLLA nanofiber membrane
图 7 纳米纤维膜接触角结果图
(a)GEL膜；(b)GEL@PLLA膜；(c)PLLA膜；(d)纳米纤维膜接触角量化结果
Figure 7. Contact Angle Results of Nanofiber Membrane
(a) GEL membrane; (b) GEL@PLLA Membrane; (c) PLLA membrane; (d) Quantitative results of contact angle of nanofiber membrane
图 8 BMSC在纳米纤维膜上的形态
(a)纳米纤维膜×1000；(b)纳米纤维膜×3000；(c)纳米纤维膜×10000；
Figure 8. Morphology of BMSC on nanofiber membrane
(a) Nanofiber membrane × 1000； (b) Nanofiber membrane × 3000； (c) Nanofiber membrane × 10000；
图 9 纯GEL膜、纯PLLA膜和GEL@PLLA膜的CCK-8检测结果
Figure 9. Pure GEL membrane, pure PLLA membrane, and GEL@PLLA CCK-8 detection results of membrane
表 1 明胶(GEL)@左旋聚乳酸(PLLA)静电纺丝工艺参数表
Table 1. gelatin (GEL)@poly (L-lactic acid) (PLLA) Electrospinning Process Parameters Table
Sample number Velocity of flow
(core/shell)/
(mm·s−1)Voltage/kV Needle-collector
distance/cmS1 1∶3 17 15 S2 1∶4 17 15 S3 1∶5 17 15 S4 1∶4 19 15 S5 1∶4 15 15 S6 1∶4 17 10 S7 1∶4 17 20 
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表 2 纤维的断裂伸长率、拉伸强度及断裂伸长率
Table 2. Elongation at break, tensile strength, and elongation at break of fibers
Sample Elongation at break /% Tensile strength /MPa GEL 10.3383 1.7544 PLLA 9.2204 2.2772 GEL@PLLA 18.2957 1.8158
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