Abstract: With the continuous development of communication technology and mobile electronic devices, electromagnetic interference problem is increasingly prominent, so the development of high performance electromagnetic shielding materials has become an important research direction. In this paper, a high conductivity MXene/polypyrrole (PPy)@bacterial cellulose (BC) film (MPB) with high conductivity was successfully prepared by a simple vacuum filtration method. The influence of different mass ratios of MXene and PPy@BC on the conductivity, mechanical properties and electromagnetic shielding performance of the composite film was deeply studied. The results show that when the mass ratio of MXene and PPy@BC is 3∶1, the conductivity and X-band electromagnetic shielding efficiency (EMI SE) reach the maximum, 1209 S/cm and 63.89 dB, respectively. In addition, due to the rich hydrogen bond interaction between PPy@BC and MXene, the maximum tensile strength of the composite film can reach 24.73 MPa, which is nearly 10 times higher than that of pure MXene film. The excellent comprehensive performance of MPB film shows its great potential in the EMI shielding of the next generation of intelligent and wearable electronic products.