Preparation and antibacterial properties of ZnFe2O4@Ag nanocomposites
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摘要: 由于传统抗生素和抗菌剂的过度使用,导致大量耐药菌滋生,对社会公共安全和人类健康产生严重威胁。因此,迫切需要开发新一代的抗菌材料来应对耐药菌危害。本研究以三氯化铁(FeCl3)、氯化锌(ZnCl2)和醋酸钠(NaOAc)为原料,采用“一锅法”合成磁性ZnFe2O4纳米微球,并把平均粒径尺寸为8.8 nm的银纳米颗粒(Ag NPs)吸附到ZnFe2O4表面,制备得到ZnFe2O4@Ag磁性纳米复合材料。该材料可有效防止Ag NPs的团聚,同时小粒径的纳米银可大幅度提升复合材料的抑菌活性,且Zn和Fe元素的引入可提升生物相容性。利用TEM、XPS、XRD、UV-Vis、FT-IR以及VSM等对复合材料进行系统表征。以革兰氏阴性菌大肠杆菌(E. coli)、革兰氏阳性金黄色葡萄球菌(S. aureus)为测试菌,研究复合材料的抑菌活性和抑菌机制。实验结果表明,ZnFe2O4@Ag在浓度为200 μg/mL时,60 min内对E. coli和S. aureus的抑菌活性可达到99.9%,抑菌机制显示,ZnFe2O4@Ag破坏细菌细胞壁与细胞膜,使得细菌内容物以及离子泄露,从而使细菌渗透压失衡,导致细菌死亡。同时该复合材料的生物相容性较Ag NPs也大幅度提升。
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关键词:
- 纳米材料 /
- ZnFe2O4@Ag /
- 抑菌 /
- 抑菌机制 /
- 磁分离
Abstract: The overuse of traditional antibiotics and antimicrobial agents has led to the growth of a large number of drug-resistant bacteria, which represents a serious threat to public safety and human health. Therefore, there is an urgent need to develop new-generation antimicrobial materials to cope with the hazards posed by drug-resistant bacteria. In this study, magnetic ZnFe2O4 nanorods were synthesised using ferric chloride (FeCl3), zinc chloride (ZnCl2) and sodium acetate (NaOAc) as raw materials. Silver nanoparticles (Ag NPs) with an average particle size of 8.8 nm were then adsorbed onto the surface of ZnFe2O4, resulting in the formation of ZnFe2O4@Ag magnetic nanocomposites.This material can effectively prevent the agglomeration of Ag NPs, while the small diameter of the nanoparticles significantly enhances the antibacterial activity of the composite material, and the introduction of Zn and Fe elements can improve biocompatibility. The composite material was systematically characterized using TEM, XPS, XRD, UV-Vis, FT-IR, and VSM, among others. The antibacterial activity and mechanism of the composite material were studied against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) as test bacteria. Experimental results show that ZnFe2O4@Ag has an antibacterial activity of 99.9% against E. coli and S. aureus within 60 minutes at a concentration of 200 μg/mL. The mechanism of antibacterial action indicates that ZnFe2O4@Ag disrupts the bacterial cell walls and membranes, causing leakage of bacterial contents and ions, thereby leading to osmotic imbalance and resulting in bacterial death. At the same time, the biocompatibility of this composite material is significantly improved compared to Ag NPs alone.-
Key words:
- Nanomaterials /
- ZnFe2O4@Ag /
- Bacteriostasis /
- Inhibition mechanism /
- Magnetic separation
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表 1 溶剂、Ag NPs、ZnFe2O4、ZnFe2O4@Ag对E. coli、S. aureus的抑菌圈尺寸
Table 1. Solvent, Ag NPs, ZnFe2O4, and ZnFe2O4@Ag on E. coli, S. aureus Circle of Inhibition Size
Bacterial Concentration/ (μg·mL-1) inhibition zones/cm(±0.05) H2O Ag ZnFe2O4 ZnFe2O4@Ag E. coli 50 0.6 0.65 0.6 0.8 100 0.6 0.75 0.6 1.0 200 0.6 1.0 0.6 1.4 400 0.6 1.3 0.6 1.7 S. aureus 50 0.6 0.6 0.6 0.7 100 0.6 0.7 0.6 1.0 200 0.6 0.9 0.6 1.2 400 0.6 1.1 0.6 1.5 -
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