ZnFe<sub>2</sub>O<sub>4</sub>@Ag纳米复合材料的制备及其抑菌性能

张艳艳; 房宇; 张萌萌; 张滢; 吴鹏; 魏艳妮; 刘晓琴; 刘嘉华; 丁佳睿; 谭玉婷; 郭少波

ZnFe₂O₄@Ag纳米复合材料的制备及其抑菌性能

1.
汉中职业技术学院药学院，陕西汉中 723001
2.
陕西理工大学生物科学与工程学院，陕西汉中 723000
3.
陕西理工大学化学与环境科学学院, 陕西汉中 723000

基金项目: 陕西省教育厅专项科研计划项目(19JK0050).

详细信息

通讯作者:
房宇，讲师，研究方向为中药药理、天然药物提取和微生物检测　E-mail: www.fyan@163.com

郭少波，副教授，研究方向为抑菌材料　E-mail：545366954@qq.com

中图分类号: O614; TB333
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- 被引次数: 0
出版历程
- 收稿日期: 2024-04-26
- 修回日期: 2024-05-22
- 录用日期: 2024-05-31
- 网络出版日期: 2024-06-22

Preparation and antibacterial properties of ZnFe₂O₄@Ag nanocomposites

1.
School of Pharmacy, Hanzhong Vocational and Technical College, Hanzhong 723001, Shaanxi, China
2.
College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
3.
School of Chemistry and Environmental Science, Shaanxi University of Technology, Hanzhong 723000, China

Funds: Special Scientific Research Project of Shaanxi Provincial Education Department (19JK0050)

摘要

摘要: 由于传统抗生素和抗菌剂的过度使用，导致大量耐药菌滋生，对社会公共安全和人类健康产生严重威胁。因此，迫切需要开发新一代的抗菌材料来应对耐药菌危害。本研究以三氯化铁(FeCl₃)、氯化锌(ZnCl₂)和醋酸钠(NaOAc)为原料，采用“一锅法”合成磁性ZnFe₂O₄纳米微球，并把平均粒径尺寸为8.8 nm的银纳米颗粒(Ag NPs)吸附到ZnFe₂O₄表面，制备得到ZnFe₂O₄@Ag磁性纳米复合材料。该材料可有效防止Ag NPs的团聚，同时小粒径的纳米银可大幅度提升复合材料的抑菌活性，且Zn和Fe元素的引入可提升生物相容性。利用TEM、XPS、XRD、UV-Vis、FT-IR以及VSM等对复合材料进行系统表征。以革兰氏阴性菌大肠杆菌(E. coli)、革兰氏阳性金黄色葡萄球菌(S. aureus)为测试菌，研究复合材料的抑菌活性和抑菌机制。实验结果表明，ZnFe₂O₄@Ag在浓度为200 μg/mL时，60 min内对E. coli和S. aureus的抑菌活性可达到99.9%，抑菌机制显示，ZnFe₂O₄@Ag破坏细菌细胞壁与细胞膜，使得细菌内容物以及离子泄露，从而使细菌渗透压失衡，导致细菌死亡。同时该复合材料的生物相容性较Ag NPs也大幅度提升。
- 纳米材料 /
- ZnFe₂O₄@Ag /
- 抑菌 /
- 抑菌机制 /
- 磁分离
Abstract: The overuse of traditional antibiotics and antimicrobial agents has led to the growth of a large number of drug-resistant bacteria, which represents a serious threat to public safety and human health. Therefore, there is an urgent need to develop new-generation antimicrobial materials to cope with the hazards posed by drug-resistant bacteria. In this study, magnetic ZnFe₂O₄ nanorods were synthesised using ferric chloride (FeCl₃), zinc chloride (ZnCl₂) and sodium acetate (NaOAc) as raw materials. Silver nanoparticles (Ag NPs) with an average particle size of 8.8 nm were then adsorbed onto the surface of ZnFe₂O₄, resulting in the formation of ZnFe₂O₄@Ag magnetic nanocomposites.This material can effectively prevent the agglomeration of Ag NPs, while the small diameter of the nanoparticles significantly enhances the antibacterial activity of the composite material, and the introduction of Zn and Fe elements can improve biocompatibility. The composite material was systematically characterized using TEM, XPS, XRD, UV-Vis, FT-IR, and VSM, among others. The antibacterial activity and mechanism of the composite material were studied against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) as test bacteria. Experimental results show that ZnFe₂O₄@Ag has an antibacterial activity of 99.9% against E. coli and S. aureus within 60 minutes at a concentration of 200 μg/mL. The mechanism of antibacterial action indicates that ZnFe₂O₄@Ag disrupts the bacterial cell walls and membranes, causing leakage of bacterial contents and ions, thereby leading to osmotic imbalance and resulting in bacterial death. At the same time, the biocompatibility of this composite material is significantly improved compared to Ag NPs alone.
- Nanomaterials /
- ZnFe₂O₄@Ag /
- Bacteriostasis /
- Inhibition mechanism /
- Magnetic separation

HTML全文

图 1 ZnFe₂O₄ @Ag纳米复合材料的制备流程图

Figure 1. Preparation process diagram of ZnFe₂O₄@Ag nanocomposite material.

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图 2 ZnFe₂O₄(a)、ZnFe₂O₄@Ag(b)的透射电镜图像及粒径分布图；ZnFe₂O₄@Ag的EDS面扫图(c-i)

Figure 2. Transmission electron microscopy images and particle size distribution of ZnFe₂O₄ (a) and ZnFe₂O₄@Ag (b); EDS surface sweeps of ZnFe₂O₄@Ag (c-i)

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图 3 ZnFe₂O₄、ZnFe₂O₄@Ag纳米复合材料XPS(a-e)、VSM(f)、UV-Vis(g)、XRD(h)表征、毒理性(i)及ROS(j,k)检测实验结果

Figure 3. ZnFe₂O4, ZnFe₂O₄@Ag nanocomposites XPS (a-e), VSM (f), UV-Vis (g), XRD (h) characterization, toxicity (i) and ROS(j,k) detection experimental results

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图 4 不同材料对E. coli、S. aureus的滤纸片扩散照片

Figure 4. Photographs of diffusion of different materials on filter paper sheets of E. coli, S. aureus

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图 5 ZnFe₂O₄@Ag纳米复合材料菌落计数分布图及具体抑菌率数据

Figure 5. Colony count distribution diagram and specific antibacterial rate data for ZnFe₂O₄@Ag nanocomposite

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图 6 ZnFe₂O₄@Ag纳米复合材料Zeta电位(a) 及离子泄露(b、c、d)实验结果

Figure 6. The following figures present the experimental results of the zeta potential (a) and ion leakage (b, c, d) of ZnFe₂O₄@Ag nanocomposites.

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图 7 ZnFe₂O₄@Ag纳米复合材料的PI染色(a、b)及细胞质物质泄露(c、d)实验结果

Figure 7. Experimental results of PI staining (a, b) and cytoplasmic content leakage (c, d) of ZnFe₂O₄@Ag nanoparticle composite material

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图 8 ZnFe₂O₄ @Ag纳米复合材料抑菌机制图

Figure 8. Diagram of antibacterial mechanism of ZnFe₂O₄@Ag nanocomposite

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表 1 溶剂、Ag NPs、ZnFe₂O₄、ZnFe₂O₄@Ag对E. coli、S. aureus的抑菌圈尺寸

Table 1. Solvent, Ag NPs, ZnFe₂O₄, and ZnFe₂O₄@Ag on E. coli, S. aureus Circle of Inhibition Size

Bacterial	Concentration/ (μg·mL^-1)	inhibition zones/cm(±0.05)
Bacterial	Concentration/ (μg·mL^-1)	H₂O	Ag	ZnFe₂O₄	ZnFe₂O₄@Ag
E. coli	50	0.6	0.65	0.6	0.8
	100	0.6	0.75	0.6	1.0
	200	0.6	1.0	0.6	1.4
	400	0.6	1.3	0.6	1.7
S. aureus	50	0.6	0.6	0.6	0.7
	100	0.6	0.7	0.6	1.0
	200	0.6	0.9	0.6	1.2
	400	0.6	1.1	0.6	1.5

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