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静电喷雾法一步制备自显影Fe3O4@壳聚糖载药栓塞微球

黄超键 谢德明

黄超键, 谢德明. 静电喷雾法一步制备自显影Fe3O4@壳聚糖载药栓塞微球[J]. 复合材料学报, 2023, 40(12): 6766-6773. doi: 10.13801/j.cnki.fhclxb.20230323.002
引用本文: 黄超键, 谢德明. 静电喷雾法一步制备自显影Fe3O4@壳聚糖载药栓塞微球[J]. 复合材料学报, 2023, 40(12): 6766-6773. doi: 10.13801/j.cnki.fhclxb.20230323.002
HUANG Chaojian, XIE Deming. One-step preparation of self-imaging Fe3O4@chitosan drug-loaded embolic microspheres by electrostatic spraying[J]. Acta Materiae Compositae Sinica, 2023, 40(12): 6766-6773. doi: 10.13801/j.cnki.fhclxb.20230323.002
Citation: HUANG Chaojian, XIE Deming. One-step preparation of self-imaging Fe3O4@chitosan drug-loaded embolic microspheres by electrostatic spraying[J]. Acta Materiae Compositae Sinica, 2023, 40(12): 6766-6773. doi: 10.13801/j.cnki.fhclxb.20230323.002

静电喷雾法一步制备自显影Fe3O4@壳聚糖载药栓塞微球

doi: 10.13801/j.cnki.fhclxb.20230323.002
详细信息
    通讯作者:

    谢德明,博士,副教授,硕士生导师,研究方向为生物医学材料与组织工程 E-mail: bme2004@126.com

  • 中图分类号: R730.8;TB383.1;TB333

One-step preparation of self-imaging Fe3O4@chitosan drug-loaded embolic microspheres by electrostatic spraying

  • 摘要: 采用静电喷雾法一步制备包裹着Fe3O4纳米粒子的壳聚糖复合微球(Fe3O4@CS微球),实现Fe3O4纳米粒子与微球同时合成。还可以按需制备粒径范围为90~1000 μm的Fe3O4@CS微球,以满足不同部位血管的临床栓塞要求。SEM显示微球形貌均匀且粒径分布均一((94±3) μm),体外降解实验证明了微球具有生物可降解性,磁共振成像测试表明所制备的Fe3O4@CS微球具有良好的临床成像能力,血液、细胞相容性评估证实Fe3O4@CS微球具有良好的生物相容性。负载盐酸阿霉素(DOX)的载药微球显示出典型的药物缓释曲线,72 h内DOX的累计释放率为28.82%。结果表明,这一步可控制备的自显影栓塞剂在经导管动脉栓塞术(TACE)未来应用中展示了巨大的潜力。

     

  • 图  1  (a) Fe3O4@壳聚糖(CS)微球的SEM图像及粒径分布直方图;((b), (c)) 微球溶胀前后的光学显微镜图及粒径分布直方图

    Figure  1.  (a) SEM image and histogram of particle size distribution of Fe3O4@chitosan (CS) microspheres; ((b), (c)) Optical microscope images of Fe3O4@CS microspheres before and after swelling and particle size distribution histograms

    图  2  不同参数对Fe3O4@CS微球直径的影响:(a) 电压;(b) 针头大小;(c) 注射速度

    Figure  2.  Effect of different parameters on Fe3O4@CS microsphere diameter: (a) Voltage; (b) Needle size; (c) Injection speed

    图  3  Fe3O4@CS微球的XRD图谱

    Figure  3.  XRD patterns of Fe3O4@CS microsphere

    NPs—Nanoparticles

    图  4  Fe3O4@CS微球截面SEM-EDX图像

    Figure  4.  SEM-EDX images of Fe3O4@CS microsphere cross-section

    图  5  Fe3O4@CS微球的体外降解图

    Figure  5.  In vitro degradation diagram of Fe3O4@CS microspheres

    图  6  (a) Fe3O4@CS微球在磁场存在下的迁移图;(b) Fe3O4@CS微球的磁滞曲线图;(c) Fe3O4@CS微球体外模型的T2加权核磁共振成像(MRI)图像

    Figure  6.  (a) Migration diagram of Fe3O4@CS microspheres in the presence of a magnetic field; (b) Hysteresis curve diagram of Fe3O4@CS microspheres; (c) T2-weighted magnetic resonance imaging (MRI) images of Fe3O4@CS microspheres in a vitro model

    图  7  不同浓度Fe3O4@CS微球的溶血率(插图1、2、3、4、5为阳性对照、阴性对照、1、5、20 mg/mL微球)

    Figure  7.  Hemolysis rate of various Fe3O4@CS microsphere contents (Insets 1, 2, 3, 4 and 5 were positive controls, negative controls, 1 mg/mL, 5 mg/mL and 20 mg/mL microspheres, respectively)

    图  8  不同浓度Fe3O4@CS微球对人脐静脉内皮细胞(HUVEC)的细胞毒性

    Figure  8.  cytotoxicity of various Fe3O4@CS microsphere concentrations on human umbilical vein endothelial cell (HUVEC) cells

    图  9  (a) 载药Fe3O4@CS微球的荧光成像图;(b) 载药Fe3O4@CS微球的载药率;(c) 载药Fe3O4@CS微球的药物体外释放;(d) 载药Fe3O4@CS微球孵育HepG2细胞24 h和48 h的细胞活力;(e) HepG2癌细胞的活/死染色荧光成像测定

    Figure  9.  (a) Fluorescence imaging of drug-loaded Fe3O4@CS microspheres; (b) Drug-loading capacities; (c) Drug release in vitro of drug-loaded Fe3O4@CS microspheres; (d) Cell viability of HepG2 cells incubating with drug-loaded Fe3O4@CS microspheres at 24 h and 48 h; (e) Live/dead staining fluorescence imaging assay of HepG2 cancer cells

    DOX—Doxorubicin hydrochloride; AM—Calcein-AM; PI—Propidium iodide; Q—Diffusion flux; R2—Correlation coefficient

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出版历程
  • 收稿日期:  2023-02-15
  • 修回日期:  2023-03-15
  • 录用日期:  2023-03-17
  • 网络出版日期:  2023-03-24
  • 刊出日期:  2023-12-01

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