Preparation and release properties of functional emulsion based on hyaluronic acid self-assembled colloidal particles
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摘要:
近年来，功能乳液因含有某种特定功能的功能因子，引起人们广泛的兴趣。多重乳液是是功能乳液的一种，含有一种或多种功能因子，在一定程度上可实现对水溶性或脂溶性功能因子的缓释。但是，多重乳液需分步制备，制备过程复杂，难以大规模生产；多重乳液属于热力学不稳定体系，乳液滴间易发生宏观聚合，导致乳液稳定性较差；缺乏能长期稳定乳液的食品级乳化剂，在食品应用领域受限。本文以大分子自组装技术制备自组装胶体粒子，在生物大分子透明质酸和溶菌酶自组装过程中引入水溶性功能因子(微量金属Zn2+)。以自组装胶体粒子为颗粒乳化剂，稳定含脂溶性功能因子(维生素D3)的大豆油，得到功能Pickering乳液。制备的乳液对水溶性和脂溶性功能因子均具有较好的缓释性能。本文给出了一种功能乳液制备的新方法，制备的乳液在食品、医药和化妆品领域具有潜在的应用。 功能乳液制备方法及功能因子缓释示意图 Abstract: Functional emulsion is one of the hot topics because of their functional factors. Bio-macromolecules hyaluronic acid (HA), lysozyme (Lys) and trace metal element zinc can self-assemble to prepare Lys-Zn2+/HA colloidal particles by electrostatic interaction. The effects of different raw material concentration on the properties of colloidal particles were studied to obtain colloidal nanoparticles under optimal assembly conditions. The size and morphology of the colloidal particles were characterized by nanometer particle size analyzer and scanning electron microscope. The results show that the formed colloidal particles have a spherical structure with a particle size of about 300 nm. The colloidal particles have surface activity and can be reassembled at the oil (containing fat-soluble vitamin D3)-water interface to stabilize oil-in-water functional Pickering emulsions. The effects of pH and salt concentration on the properties and emulsifying properties of colloidal particles were investigated in detail. The sustained release properties of the emulsion to trace metals and vitamin D3 functional factors were studied with the optimum emulsion performance. The results show that the emulsion has a certain sustained-release performance for both water-soluble and fat-soluble functional factors and has potential applications in the fields of food, medicine and cosmetics.-
Key words:
- self-assembly /
- colloidal nanoparticles /
- bio-macromolecule /
- functional emulsion /
- sustained-release
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图 1 维生素D3(VD3)-溶菌酶(Lys)-Zn2+/生物大分子透明质酸(HA)功能乳液制备方法及功能因子缓释示意图
Figure 1. Schematic illustration of preparation method of vitamin D3 (VD3)- lysozyme (Lys)-Zn2+/Bio-macromolecules hyaluronic acid (HA) functional emulsion and slow release of functional factors
图 2 Lys-Zn2+/HA胶体粒子的粒径，ζ电位和多分散度随Lys浓度的变化
Figure 2. Variation of particle size, ζ-potential and polydispersity of Lys-Zn2+/HA colloidal particles with Lys concentration
图 3 Lys-Zn2+/HA胶体粒子的粒径分布(a)和SEM图(b)
Figure 3. Particle size distribution (a) and SEM image (b) of Lys-Zn2+/HA colloidal particles
图 4 Lys-Zn2+/HA胶体粒子粒径，ζ电位和多分散度随pH的变化，胶体粒子的浓度为1 mg/mL
Figure 4. Variation of Lys-Zn2+/HA colloidal particle size，ζ-potential and polydispersity with pH, the concentration of colloidal particles is 1 mg/mL
图 5 不同pH值VD3-Lys-Zn2+/HA乳液数码照片和乳液滴的显微镜照片(a)；乳液滴平均粒径和多分散度随pH的变化(b)；乳液滴平均粒径随时间的变化(c)。乳液为制备一天的乳液。胶体粒子的浓度为1 mg/mL
Figure 5. Digital photographs of VD3-Lys-Zn2+/HA emulsions and microscope photographs of emulsion droplets with different pH (a); average particle diameter and polydispersity of emulsion droplets as a function of pH (b); average particle diameter of emulsion droplets as a function of time (c). The emulsions were incubated 1 day after homogenization. The concentration of colloidal particles is 1 mg/mL
图 6 Lys-Zn2+/HA胶体粒子粒径, ζ电位和多分散度随盐浓度的变化，pH 5.1，胶体粒子的浓度为1 mg/mL
Figure 6. Variation of Lys-Zn2+/HA colloidal particle size, ζ-potential and polydispersity with salt concentration, pH 5.1, the concentration of colloidal particles is 1 mg/mL
图 7 不同盐浓度VD3-Lys-Zn2+/HA乳液数码照片和乳液滴的显微镜照片(a)；乳液滴平均粒径和多分散度随盐浓度的变化(b)；乳液滴平均粒径随时间的变化(c)。乳液为制备一天的乳液。pH 5.1，胶体粒子的浓度为1 mg/mL
Figure 7. Digital photographs of VD3-Lys-Zn2+/HA emulsions and microscope photographs of emulsion droplets with different salt concentrations (a); average particle diameter and polydispersity of emulsion droplets as a function of salt concentration (b); average particle diameter of emulsion droplets as a function of time (c). The emulsions were incubated 1 day after homogenization. pH 5.1, the concentration of colloidal particles is 1 mg/mL
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