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多孔材料ZIF-8的制备及其对布洛芬的载药性能

孙瑞华 杨卓凡 张芮博 江奇 卢晓英 廖海

孙瑞华, 杨卓凡, 张芮博, 等. 多孔材料ZIF-8的制备及其对布洛芬的载药性能[J]. 复合材料学报, 2024, 42(0): 1-8.
引用本文: 孙瑞华, 杨卓凡, 张芮博, 等. 多孔材料ZIF-8的制备及其对布洛芬的载药性能[J]. 复合材料学报, 2024, 42(0): 1-8.
SUN Ruihua, YANG Zhuofan, ZHANG Ruibo, et al. Preparation of the porous ZIF-8 and the behaviors of loading ibuprofen[J]. Acta Materiae Compositae Sinica.
Citation: SUN Ruihua, YANG Zhuofan, ZHANG Ruibo, et al. Preparation of the porous ZIF-8 and the behaviors of loading ibuprofen[J]. Acta Materiae Compositae Sinica.

多孔材料ZIF-8的制备及其对布洛芬的载药性能

基金项目: 国家自然科学基金(51602266); 四川省重点研发项目(2021YFG2016); 中央引导地方科技发展资金面上项目(2021ZYD0066);成都市技术创新研发项目(2022-YF05-00320-SN); 大学生创新创业训练计划(SRTP2023187)。
详细信息
    通讯作者:

    卢晓英,博士,副教授,硕士生导师,研究方向为生物材料方向 E-mail: luxy2005@swjtu.cn

    廖 海,博士,副教授,博士生导师,研究方向为植物基因方向 E-mail: ddliaohai@swjtu.cn

  • 中图分类号: R318.08

Preparation of the porous ZIF-8 and the behaviors of loading ibuprofen

Funds: National Natural Science Foundation of China (51602266); Key R&D Project of Sichuan Province (2021YFG2016); Top-level Project of Central-Guided Local Science and Technology Development Funds (2021ZYD0066); Chengdu Technological Innova-tion Research and Development Project (2022-YF05-00320-SN); Innovation and Entrepreneurship Training Program for College Students (SRTP2023187).
  • 摘要: 由于难溶性药物低的溶解度,严重限制了其口服生物利用度。对此,结合金属—有机框架材料(MOFs)具有疏水性和高比表面积的特点,以溶液共沉淀法制备的MOF多孔材料ZIF-8作为载药基底。通过改变Zn2+与2-甲基咪唑(2-Methylimidazole)的摩尔比,进而对ZIF-8的比表面积及孔径分布进行调控,然后对难溶性药物布洛芬(IBP)的负载性能进行研究。研究结果表明:当Zn2+与2-甲基咪唑的摩尔比为1∶8时,材料ZIF-8(8)具有最大的比表面积和孔体积,分别为1187 m2/g和1.183 cm3/g,其对难溶性药物布洛芬的负载量高达21.8%。且药物载体复合材料IBP-ZIF-8(8)表现出良好的体外溶出度,在pH为2.5和7.4的磷酸缓冲溶液中,其累积溶出度均为98%左右。ZIF-8(8)处理的RAW246.7细胞存活率均高达94%以上,表现出良好的生物安全性。

     

  • 图  1  不同ZIF-8材料的XRD衍射图(a) 和红外图谱(b)

    Figure  1.  XRD spectrum (a) and FTIR spectrum (b) of ZIF-8 materials

    图  2  不同ZIF-8材料的SEM照片

    Figure  2.  SEM images of ZIF-8 materials

    图  3  不同ZIF-8材料的N2吸脱附曲线(a);DFT孔径曲线(b);BJH孔径曲线(c);HK孔径曲线(d)

    Figure  3.  N2 adsorption and desorption curves (a); DFT pore size curves (b); BJH pore size curves (c); HK pore size curves (d) of ZIF-8 materials

    图  4  布洛芬在乙醇溶剂和不同pH下(pH=7.4;pH=2.5)的紫外吸收光谱

    Figure  4.  UV absorption spectra of ibuprofen in ethanol solvent and at different pH values (pH=7.4; pH=2.5)

    图  5  布洛芬在在乙醇溶剂和不同pH下(pH=7.4; pH=2.5)的标准曲线

    Figure  5.  Standard curves of ibuprofen in ethanol solvent and at different pH values (pH=7.4; pH=2.5)

    图  6  IBP与IBP-ZIF-8(8)的XRD衍射图谱(a)和IBP、IBP-ZIF-8(8)和ZIF-8(8)的TG图谱(b)

    Figure  6.  XRD specrum of IBP and IBP-ZIF-8(8) (a) and TG curves of IBP, IBP-ZIF-8(8) and ZIF-8(8) (b)

    图  7  IBP-ZIF-8(8)在不同pH下的溶出曲线(a:pH=2.5; b:pH=7.4)

    Figure  7.  Dissolution curves of IBP-ZIF-8(8) at different pH values (a: pH=2.5; b: pH=7.4)

    图  8  RAW246.7细胞与ZIF-8(8)共培养24 h后的细胞存活率

    Figure  8.  Survival rate of RAW246.7 cells co-cultured with ZIF-8(8) for 24 hours.

    表  1  实验过程中使用的试剂

    Table  1.   Reagents used during the experiment

    Name of reagentsPuritySource
    Zinc nitrate hydrateARKelong Chemical Reagent Co.
    EthanolARKelong Chemical Reagent Co.
    2-MethylimidazoleARZhenri Chemical Co.
    Ibuprofen (IBP)ARSolarbio Science & Technology Co.
    Phosphate solutionARPricella Biotechnology Co.
    下载: 导出CSV

    表  2  不同ZIF-8材料的比表面积和孔径参数

    Table  2.   Specific surface area and pore size parameters of ZIF-8 materials

    Samples SBET/
    (m2·g−1)
    Vmesopore/
    (cm3·g−1)
    Vmicropore/
    (cm3·g−1)
    ZIF-8(4) 872.033 0.199 0.615
    ZIF-8(8) 1186.919 0.305 0.878
    ZIF-8(12) 1078.442 0.221 0.668
    Notes: SBET: Specific surface area; Vmesopore: mesopore volume; Vmicropore: micropore volume
    下载: 导出CSV

    表  3  不同ZIF-8材料对布洛芬的载药量

    Table  3.   Loading capacity of ZIF-8 material for ibuprofen

    SamplesSolventDrug loading/%
    ZIF-8(4)ethanol11.9
    ZIF-8(8)ethanol21.8
    ZIF-8(12)ethanol18.2
    下载: 导出CSV
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出版历程
  • 收稿日期:  2024-01-17
  • 修回日期:  2024-02-18
  • 录用日期:  2024-03-01
  • 网络出版日期:  2024-04-10

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