蒲公英提取物-茶多酚-玉米秸秆纤维素抗菌复合膜制备与性能

张群利; 邬泽凯; 崔琳琳; 谢文静; 于启蒙; 刘宇鹏

蒲公英提取物-茶多酚-玉米秸秆纤维素抗菌复合膜制备与性能

1.
东北林业大学　工程技术学院, 黑龙江哈尔滨 150040
2.
哈尔滨商业大学　药学院, 黑龙江哈尔滨 150076
3.
森林持续经营与环境微生物工程黑龙江省重点实验室, 黑龙江哈尔滨 150040

基金项目: 中央高校基本科研业务费专项资金资助(2572019 BL01)

详细信息

通讯作者:
张群利，博士，副教授，硕士生导师，研究方向为功能性包装材料　E-mail: zhangqunli@nefu.edu.cn

中图分类号: TS71+1;TB332
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- 被引次数: 0
出版历程
- 收稿日期: 2022-10-19
- 修回日期: 2022-11-22
- 录用日期: 2022-12-09
- 网络出版日期: 2022-12-22

Preparation and Performance of Cellulose Antibacterial Composite Film from Corn Stover with Dandelion Extract and Tea Polyphenols

1.
College of Engineering and Technology, Northeast Forestry University, Harbin 150040, Heilongjiang, China
2.
School of Pharmacy, Harbin University of Commerce, Harbin 150076, Heilongjiang, China
3.
Key Laboratory of Sustainable Forest Management & Environmental Microbiology in Heilongjiang Province, Harbin 150040, Heilongjiang, China

Funds: Fundamental Research Funds for the Central Universities (2572019 BL01)

摘要

摘要: 纤维素是世界上最丰富的天然有机物质，以纤维素为原料制备的绿色环保抗菌材料，具有良好的力学性能、生物相容性、可降解性、热稳定性和抗菌性能。农业废弃物秸秆也是纤维素的来源之一，玉米秸秆的高附加值利用不仅能减轻因焚烧和丢弃带来的污染和资源浪费，还对探索秸秆的工业化利用和农业可持续发展具有深远的意义。本文以玉米秸秆制备的微晶纤维素(CSMCC)为原料，1-丁基-3-甲基咪唑氯盐([Bmim]Cl)为溶剂体系，蒲公英提取物(DE)和茶多酚(TP)为抗菌剂，通过共混法制备抗菌复合膜。通过FTIR、XRD、SEM和热重分析对复合膜的形貌和结构进行表征及对力学、光学、阻隔、抑菌等性能测试分析。结果表明，DE和TP与纤维素基膜较好地复合，复配抗菌剂DE-TP抗菌复合膜相比于DE、TP抗菌复合膜具有较优的拉伸强度(52.60±6.33 MPa)、氧气阻隔性能〔氧气透过系数为1.65±0.25×10^-11 cm³·cm/(cm²·s·Pa)〕，且对大肠杆菌和金黄色葡萄球菌具有更好的抑制作用。同时DE/TP抗菌复合膜断裂伸长率相比于纤维素基膜提升了53.96%，透光率保持在82.56±0.26%，具有良好的力学性能和光学性能，为制备对控制食品腐败变质和生物资源高效利用具有重要意义的环境友好型抗菌复合膜的研发提供新思路。1CSRGC 0#(a)和1#(b)、2#(c)、3#(d)抗菌复合膜表面和断面SEM图
- 纤维素 /
- 蒲公英 /
- 茶多酚 /
- 力学性能 /
- 光学性能 /
- 阻隔性能 /
- 抑菌性能 /
- 功能材料
Abstract: The high value-added utilization of corn straw can not only reduce pollution and resource waste, but also has far-reaching significance for exploring the industrial utilization of corn straw and sustainable agricultural development. Using microcrystalline cellulose (CSMCC) prepared from corn straw as raw material, 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) as solvent system, dandelion extract (DE) and tea polyphenol (TP) as antibacterial agents, antibacterial composite film was prepared by blending method. The morphology and structure of the composite film were characterized by Fourier Transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy and thermogravimetric analysis, and the mechanical, optical, barrier and antibacterial properties were tested and analyzed. The results show that DE and TP are well combined with cellulose base film. The composite antibacterial agent DE-TP antibacterial composite film has better tensile strength (52.60±6.33 MPa), oxygen barrier performance (oxygen transmission coefficient is (1.65±0.25)×10⁻¹¹ cm³·cm/(cm²·s·Pa)) and better inhibition effect on Escherichia coli and Staphylococcus aureus than the DE and TP antibacterial composite films. At the same time, compared with the cellulose base film, elongation at break of DE-TP antibacterial composite film increased by 53.96%, and the light transmittance is (82.56±0.26)%, which has good mechanical and optical properties. It provides a new idea for the research and development of environment-friendly antibacterial composite film, which is important for controlling food spoilage and efficient utilization of biological resources.
- cellulose /
- dandelion /
- tea polyphenol /
- mechanical properties /
- optical properties /
- barrier properties /
- bacteriostatic properties /
- functional materials

HTML全文

图 1 玉米秸秆微晶纤维素(CSMCC) 0#(a)和1#(b)、2#(c)、3#(d)抗菌复合膜图

Figure 1. Pictures of microcrystalline cellulose prepared from corn straw (CSMCC) 0 # (a) and 1 # (b), 2 # (c), 3 # (d) antibacterial composite film

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图 2 抗菌复合膜制备工艺及评价

Figure 2. Preparation and evaluation of antibacterial composite film

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图 3 CSMCC和市购微晶纤维素(MCC) FTIR谱图

Figure 3. FTIR spectra of CSMCC and commercial microcrystalline cellulose (MCC)

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图 4 CSMCC和MCC XRD图

Figure 4. XRD pattern of CSMCC and MCC

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图 5 DE、TP、CSRGC和抗菌复合膜FTIR谱图

Figure 5. FTIR spectra of DE, TP, CSRGC and antibacterial composite film

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图 6 CSRGC和抗菌复合膜XRD图

Figure 6. XRD pattern of CSRGC and antibacterial composite film

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图 7 CSRGC 0#(a)和1#(b)、2#(c)、3#(d)抗菌复合膜表面SEM图

Figure 7. SEM images of surface of CSRGC 0#(a) and 1#(b), 2#(c), 3#(d) antibacterial composite film

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图 8 CSRGC 0#(a)和1#(b)、2#(c)、3#(d)抗菌复合膜断面SEM图

Figure 8. SEM images of section of CSRGC 0#(a) and 1#(b), 2#(c), 3#(d) antibacterial composite film

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图 9 CSRGC和抗菌复合膜TG曲线

Figure 9. TG diagram of CSRGC and antibacterial composite film

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图 10 CSRGC和抗菌复合膜DTG曲线

Figure 10. DTG diagram of CSRGC and antibacterial composite film

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图 11 CSRGC和抗菌复合膜的透光率和雾度

Figure 11. Light transmittance and haze of CSRGC and antibacterial composite film

Notes: Different letters represent significant differences (P＜0.05).

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表 1 抗菌复合膜组分

Table 1. Composition of antibacterial composite film

Sample	DE content/%	TP content/%	Remarks
0#	—	—	As blank group
1#	15	15	w(DE)∶w(TP)=1∶1
2#	30	—
3#	—	30
Notes: DE—Dandelion extract; TP—Tea polyphenol; "—" means not added; The added amount of DE and TP is the percentage of the mass of DE and TP in the mass of CSMCC.

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表 2 CSRGC和抗菌复合膜的失重情况

Table 2. Weight loss of antibacterial composite film

Sample	T_5%/℃	T_10%/℃	T_30%/℃	T_50%/℃
0#	271.1	276.1	288.1	299.1
1#	235.5	277.5	310.3	326.0
2#	217.0	261.9	283.6	295.6
3#	66.5	244.8	275.2	289.9
Notes: T_5%, T_10%, T_30%, T_50%—Corresponding temperature when mass loss rate is 5%, 10%, 30%, 50%.

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表 3 抗菌复合膜的力学性能

Table 3. Mechanical properties of antibacterial composite film

Sample	TS/MPa	EAB/%	EM/GPa
0#	99.35±10.56^a	8.97±0.90^c	11.08±0.64^a
1#	52.60±6.33^b	13.81±1.76^ab	3.82±0.32^b
2#	50.83±5.04^b	11.57±1.88^bc	4.49±0.94^b
3#	42.21±4.82^b	15.51±2.43^a	2.78±0.50^c
Notes: TS—Tensile strength; EAB—Elongation at break; EM—Elastic modulus; Different letters represent significant differences (P＜0.05).

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表 4 RGC/CS/AgNW抗菌复合膜阻隔性能

Table 4. Barrier properties of RGC/CS/AgNW antibacterial composite film

Sample	OTC/[cm³·cm/(cm²·s·Pa)]
0#	(3.13±0.99)×10^{−12 d}
1#	(1.65±0.25)×10^{−11 b}
2#	(2.11±0.29)×10^{−11 a}
3#	(2.22±0.11)×10^{−11 a}
Notes: OTC—Oxygen transmission coefficient; Different letters represent significant differences (P＜0.05).

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表 5 抗菌复合膜抑菌性能

Table 5. Antibacterial properties of composite film

Sample	E. coli/mm	S. aureus/mm
0#	0^d	0^d
1#	15.22±0.15^a	15.86±0.25^a
2#	11.32±0.12^c	14.29±0.25^b
3#	14.11±0.15^b	11.16±0.11^c
Notes: E. coli—Escherichia coli; S. aureus—Staphylococcus aureus; Different letters represent significant differences (P＜0.05).

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