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Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate 复合材料的协同抑菌性能

汪艳 张强 陈惠惠 邱莹 胡瑞玲 李亚鹏 赵欣鑫 安雪 刘荟芝 郭少波

汪艳, 张强, 陈惠惠, 等. Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate 复合材料的协同抑菌性能[J]. 复合材料学报, 2024, 42(0): 1-13.
引用本文: 汪艳, 张强, 陈惠惠, 等. Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate 复合材料的协同抑菌性能[J]. 复合材料学报, 2024, 42(0): 1-13.
WANG Yan, ZHANG Qiang, CHEN Huihui, et al. Synergistic antibacterial properties of Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate composites[J]. Acta Materiae Compositae Sinica.
Citation: WANG Yan, ZHANG Qiang, CHEN Huihui, et al. Synergistic antibacterial properties of Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate composites[J]. Acta Materiae Compositae Sinica.

Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate 复合材料的协同抑菌性能

基金项目: 陕西省教育厅项目(20JS015),陕西省自然科学基金(2023-YBGY-486)和陕西理工大学基础研究基金(SLGRCQD2309)
详细信息
    通讯作者:

    张强,教授,硕士生导师,研究方向为多环芳烃的合成, E-mail: zhangqiang22@126.com

  • 中图分类号: O626;TB333

Synergistic antibacterial properties of Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate composites

Funds: Project of Shaanxi Provincial Department of Education (20JS015), Shaanxi Provincial Natural Science Foundation (2023-YBGY-486), and Fundamental Research Fund of Shaanxi University of Science and Technology (SLGRCQD2309)
  • 摘要: 随着耐药细菌的快速增长,有机抑菌剂已无法满足社会公共卫生需求,高活性复合抑菌剂不仅可以保留单组分的性质,还可以显示出更加优异的抑菌性能,因而成为抑菌材料的重要研究方向。本研究通过制备纳米Cu2S材料,然后与3-(苯并噻唑-2-巯基)丙烷磺酸钠反应,制备出结构新颖的Cu2S@Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate(Cu2S@SBPF)材料,采用透射电子显微镜(TEM)、X射线衍射仪(XRD)、紫外可见分光光度计(UV-vis)、傅里叶变换红外光谱仪(FT-IR)及X射线光电子能谱分析仪(XPS)等测试手段对样品的微观形貌、结构、元素组成等进行表征,探究了该复合材料对革兰氏阴性菌大肠杆菌(E. coli)、革兰氏阳性菌金黄色葡萄球菌(S. aureus)和耐药菌沙门氏菌(T-Salmonella)的抑菌性能。结果表明,浓度为500 µg/mL的复合材料在60 min时对E. coliS. aureusT-Salmonella的抑菌率均达到99.99%且对E. coli最为敏感。抑菌机制表明,该复合材料能破坏细菌的细胞壁进入细菌内部,抑制细菌呼吸,最终使细菌死亡。这一成果有望为解决细菌耐药问题提供新的方案。

     

  • 图  1  Cu2S@SBPF复合材料的合成示意图

    Figure  1.  Illustration of the synthesis of Cu2S@SBPF composites

    SBPF—Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate

    图  2  (a) Cu2S的TEM图;(b) Cu2S的HRTEM图;(c) (d) (e) Cu2S的EDS图;

    Figure  2.  (a) TEM diagram of Cu2S; (b) HRTEM of Cu2S; EDS diagram of (c) (d) (e) Cu2S;

    图  3  (a) (b) (c) Cu2S、SBPF 、Cu2S@SBPF的XRD图、红外光谱图和紫外吸收光谱图;(d-i) Cu2S@SBPF的XPS谱图

    Figure  3.  (a) (b) (c) XRD、Infrared spectra and UV absorption spectra of Cu2S, SBPF and Cu2S@SBPF; (d-i) XPS spectra of Cu2S@SBPF

    图  4  (a) SBPF和 Cu2S的优化电子结构和SBPF的静电势(ESP)分析示意图;(b) Cu2S与SBPF的结合能

    Figure  4.  (a) Schematic diagram of the optimized electronic structures of SBPF and Cu2S and the electrostatic potential (ESP) analysis of SBPF; (b) Binding energy between Cu2S and SBPF

    图  5  不同材料对E. coliS. aureusT-Salmonella的滤纸片扩散照片;A、B、C、D 分别对应溶剂蒸馏水、Cu2S、SBPF以及 Cu2S@SBPF。图中(a1)代表浓度为0.5、1、2、5 mg/mL的不同抑菌材料对E. coli的抑菌结果照片。(b1)、(c1)为S. aureusT-Salmonella的抑菌结果照片;(a2)、(b2)、(c2)分别为不同材料对E. coliS. aureus、T-Salmonella的抑菌圈直径随浓度变化曲线

    Figure  5.  Diffusion photos of E. coli, S. aureus and T-Salmonella by different materials on filter paper; A, B, C and D correspond to the solvents distilled water, Cu2S, SBPF and Cu2S@SBPF, respectively. In the figure (a1), the antibacterial results of different antibacterial materials with concentrations of 0.5, 1, 2 and 5 mg/mL against E. coli are shown. (b1) and (c1) are photos of antibacterial results of S. aureus and T-Salmonella; (a2), (b2) and (c2) show the inhibition zone diameter curves of different materials against E. coli, S. aureus and T-Salmonella as a function of concentration, respectively.

    图  6  Cu2S@SBPF复合材料菌落计数照片

    Cu2S@SBPF复合材料抑制 E. coli(a)、S. aureus(b)和 T-Salmonella(c)的菌落计数分布图;(d)为纳米复合材料的时间-杀菌曲线图;(e)图为纳米复合材料对三种测试菌在不同时间的抑菌率比较图

    Figure  6.  Cu2S@SBPF Colony count photo of the composite

    Cu2S@SBPF Colony count distribution diagram of E. coli(a), S. aureus(b) and T-Salmonella(c) inhibited by the composite. (d) shows the time-sterilization curve of the nanocomposite; (e) Figure shows the comparison of antibacterial rates of nanocomposites against the three test bacteria at different times

    图  7  (a)Cu2S@SBPF复合材料与三种测试菌混合5和60 min后的Zeta电位值;(b)ICP-OES测得的铜阳离子累积释放图;(c)复合材料毒理性实验结果

    Figure  7.  (a) The Zeta potential values of the Cu2S@SBPF composite after mixing with the three test bacteria for 5 and 60 min; (b) Cumulative release of copper cations measured by ICP-OES; (c) experimental results of toxicity of composite materials

    图  8  Cu2S@SBPF复合材料对E. coli(d)、S. aureus(e)和 T-Salmonella(f)的 PI 染色照片,(a、b、c)为对应的纯菌对照效果图。Cu2S@SBPF复合材料作用于E. coli(g)、S. aureus(h)和 T-Salmonella (i)的细胞质泄露结果

    Figure  8.  Photos of the PI staining of the Cu2S@SBPF composite for E. coli(d), S. aureus(e) and T-Salmonella(f), and (a, b, c) are the corresponding rendering of the pure bacteria control. Results of cytoplasmic leakage of the Cu2S@SBPF composite acting on E. coli(g), S. aureus(h), and T-Salmonella (i)

    图  9  Cu2S@SBPF复合材料抑菌机制图

    Figure  9.  Cu2S@SBPF Antibacterial mechanism diagram of composite material

    表  1  溶剂、Cu2S、SBPF以及 Cu2S@SBPF对E. coliS. aureusT-Salmonella的抑菌圈尺寸

    Table  1.   Inhibition zone size of solvent, Cu2S, SBPF, and Cu2S@SBPF against E. coli, S. aureus, and T-Salmonella

    BacterialConcentration/
    (mg·mL−1)
    Inhibition zones/cm (±0.05)
    H2OCu2SSBPFCu2S@SBPF
    E. coli0.50.60.90.61
    10.61.10.71.2
    20.61.60.81.9
    50.62.112.4
    S. aureus0.50.60.60.60.7
    10.60.70.60.8
    20.61.20.61.9
    50.61.70.72.3
    T-Salmonella0.50.60.70.60.8
    10.60.80.61
    20.610.71.3
    50.61.50.91.6
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  • 收稿日期:  2024-02-28
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