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温敏聚合物修饰中空介孔二氧化硅纳米粒子及其复合纳米纤维的构建与释药性能

裴文祥 马世杰 杨浪飞 高玉洁 吴金丹

裴文祥, 马世杰, 杨浪飞, 等. 温敏聚合物修饰中空介孔二氧化硅纳米粒子及其复合纳米纤维的构建与释药性能[J]. 复合材料学报, 2024, 42(0): 1-12.
引用本文: 裴文祥, 马世杰, 杨浪飞, 等. 温敏聚合物修饰中空介孔二氧化硅纳米粒子及其复合纳米纤维的构建与释药性能[J]. 复合材料学报, 2024, 42(0): 1-12.
PEI Wenxiang, MA Shijie, YANG Langfei, et al. Construction and drug release performance of thermosensitive copolymer-modified hollow mesoporous silica and the composite nanofibers[J]. Acta Materiae Compositae Sinica.
Citation: PEI Wenxiang, MA Shijie, YANG Langfei, et al. Construction and drug release performance of thermosensitive copolymer-modified hollow mesoporous silica and the composite nanofibers[J]. Acta Materiae Compositae Sinica.

温敏聚合物修饰中空介孔二氧化硅纳米粒子及其复合纳米纤维的构建与释药性能

基金项目: 浙江省重点研发计划项目(2023C01196);浙江省自然科学基金资助项目(LQ23E030013)
详细信息
    通讯作者:

    吴金丹,博士,教授,硕士生导师,研究方向为医用纺织及涂层材料、绿色染整技术 E-mail: wujindan@zstu.edu.cn

  • 中图分类号: TB332

Construction and drug release performance of thermosensitive copolymer-modified hollow mesoporous silica and the composite nanofibers

Funds: Zhejiang Provincial Key Research and Development Program (2023C01196); Natural Science Foundation of Zhejiang Province (LQ23E030013)
  • 摘要: 传统的载药纳米纤维存在药物负载不稳定、释放过快等问题。基于此,本文利用温敏聚合物包覆中空介孔二氧化硅纳米颗粒(HMSN),将其作为药物载体与聚己内酯(PCL)纳米纤维复合,探究了复合纳米纤维膜的释药及抗菌性能。采用自由基聚合方法在HMSN表面接枝异丙基丙烯酰胺与丙烯酰胺的共聚物(P(NIPAM-co-AM)),将疏水性药物环丙沙星(CIP)负载到共聚物改性纳米粒子(PHMSN)中,利用SEM、TEM、TG、比表面积分析(BET)、FTIR及紫外-可见吸收光谱(UV-Vis)等手段表征了HMSN和PHMSN的微观结构和温度响应性能等。将PCL与载药PHMSN共混后利用静电纺丝技术制备了复合纤维膜(CIP@PHMSN-PCL)。CIP@PHMSN-PCL具有温度刺激响应的药物控释功能,在45 ℃和25 ℃下,72 h时CIP的累积释放率分别达到90.78%和72.67%。Korsmeyer-Peppas模型较好地描述了药物释放动力学,表明扩散是复合纤维膜释药的主要机制。45 ℃条件下,载药纤维膜对大肠杆菌(E. coil)和金黄色葡萄球菌(S. aureus)的抑菌率均达到100%;而在25 ℃下,膜对两种菌的抑菌率仅为92.34%和95.83%,证明了不同温度下CIP@PHMSN-PCL膜释药性能的差异。总之,载药PHMSN复合纳米纤维膜具有环境温度调控的释药功能及优异的抗菌活性,在生物医学领域具有潜在的应用价值。

     

  • 图  1  中空介孔二氧化硅纳米颗粒(HMSN)的SEM图(a)和TEM图(b)

    Figure  1.  SEM image (a) and TEM image (b) of hollow mesoporous silica nanoparticles (HMSN)

    图  2  HMSN的FTIR光谱图(a)氮气吸脱附等温线和孔径分布图(b)

    Figure  2.  FTIR spectrum of HMSN (a) Nitrogen adsorption-desorption isotherm and pore size distribution (b)

    图  3  HMSN和共聚物改性HMSN(PHMSN)的FTIR图(a)和TGA(b)图;HMSN(c)和PHMSN(d)的XPS图

    Figure  3.  FTIR images (a) and TGA images (b) of HMSN and copolymer modification HMSN (PHMSN); XPS images of HMSN (c) and PHMSN (d)

    图  4  HMSN(a)和PHMSN(b)的动态光散射(DLS)分析图;HMSN和PHMSN的SAXD图(c)PHMSN的SEM图(插入图为TEM图)(d)

    Figure  4.  Dynamic light scattering (DLS) analysis images of HMSN (a) and PHMSN (b); SAXD images of HMSN and PHMSN (c) SEM image of PHMSN (the inserted image is a TEM image) (d)

    图  5  药物与PHMSN的质量比对包封率(a)载药率(b)和影响

    Figure  5.  Effect of mass ratio of drug and PHMSN on encapsulation efficiency (a) and drug loading efficiency (b)

    图  6  两种温度下环丙沙星(CIP)@HMSN和CIP@PHMSN的释药累积量曲线(a)和CIP@PHMSN动力学拟合曲线(b)

    Figure  6.  Drug release cumulative curves (a) of ciprofloxacin (CIP)@HMSN and CIP@PHMSN and the kinetic fitting curve (b) of CIP@PHMSN at two temperatures

    图  7  PCL纤维膜和CIP@PHMSN-PCL纤维膜的FTIR图(a);PCL纤维膜的SEM图(b);CIP@PHMSN-PCL纤维膜的SEM图(c)和TEM图(d)

    Figure  7.  FTIR image (a) of PCL fiber membrane and CIP@PHMSN-PCL fiber membrane; SEM image (b) of PCL fiber membrane; SEM image (c) and TEM image (d) of CIP@PHMSN-PCL fiber membrane

    图  8  不同温度下纤维膜的释药释药累积量曲线(a)以及动力学拟合(b)

    Figure  8.  Drug release cumulative curves (a) and kinetic fitting (b) of fiber membranes at different temperatures

    图  9  不同纤维膜对E. coli(a)和S. aureus(b)的抑菌率

    Figure  9.  Antibacterial rates of different fiber membranes against E. coli (a) and S. aureus (b)

    表  1  25℃下纤维膜的释药累积量动力学拟合

    Table  1.   Kinetic fitting of cumulative drug release from fiber membrane at 25℃

    Model Equation R2
    First-Order Model Mt=59.07(1-e−0.13 t) 0.812
    Zero-Order Model Mt=0.79t+20.54 0.862
    Higuchi Model Mt=1.58(t1/2)+20.54 0.849
    Korsmeyer-Peppas Model Mt=17.24(t0.324) 0.980
    Notes:t is the time of release; Mt is the amount of drug released at time t; R2 is the regression coefficients.
    下载: 导出CSV

    表  2  45℃下纤维膜的释药累积量动力学拟合

    Table  2.   Kinetic fitting of cumulative drug release from fiber membrane at 45℃

    Model Equation R2
    First-Order Model Mt=78.66 (1-e−0.11 t) 0.848
    Zero-Order Model Mt=1.04t+26.05 0.869
    Higuchi Model Mt=2.09(t1/2)+26.04 0.858
    Korsmeyer-Peppas Model Mt=21.57(t0.434) 0.991
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-12-05
  • 修回日期:  2024-01-08
  • 录用日期:  2024-01-20
  • 网络出版日期:  2024-02-29

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