Preparation and properties of bifunctional magnetic fluorescent core-shell nanoparticles
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摘要: 采用等离子体表面改性技术对石墨烯包覆氮化铁磁性纳米颗粒 (G@FeN-MP)进行CO2等离子体改性得到氧化石墨烯包覆氮化铁磁性纳米颗粒 (GO@FeN-MP)。通过溶剂热法进一步制备了硫化锌修饰的氧化石墨烯包覆氮化铁磁性纳米颗粒 (ZnS-GO@FeN-MP),利用X射线衍射 (XRD)、X射线光电子能谱 (XPS)、透射电子显微镜 (TEM)、傅立叶变换红外光谱 (FTIR)、紫外可见光谱 (UV)、光致发光光谱 (PL)和拉曼光谱 (Raman)对复合材料进行表征,同时进行A549细胞毒性评估与ZnS-GO@FeN-MP偶联二抗的联合应用揭示了其在实验生物学功能中的潜在应用,研究结果确认了该纳米复合材料的优良生物相容性。Abstract: Graphene coated ferronitride magnetic nanoparticles (G@FeN-MP) were modified by CO2 plasma surface modification technology to obtain graphene oxide coated ferronitride magnetic nanoparticles (GO@FeN-MP). Zinc sulfide modified graphene oxide coated ferronitride magnetic nanoparticles (ZnS-GO@FeN-MP) were prepared by solvothermal method, and the composites were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet visible spectroscopy (UV), photoluminescence spectroscopy (PL) and Raman spectroscopy (Raman), and concurrent cytotoxicity assessment of A549 cells and the coupled application of ZnS-GO@FeN-MP secondary antibodies have unveiled their potential in experimental biological functions, with study results affirming the excellent biocompatibility of this nanocomposite material.
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Key words:
- Core-shell magnetic nanoparticles /
- plasma /
- fluorescence /
- ZnS /
- biocompatibility
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图 4 G@FeN-MP、GO@FeN-MP、ZnS-GO@FeN-MP-1和ZnS-GO@FeN-MP-2的(a)全谱图 和(b) C 1s高分辨XPS谱图; ZnS-GO@FeN-MP-1和ZnS-GO@FeN-MP-2的(c) Zn 2p的高分辨XPS谱图和(d) S 2p的高分辨XPS谱图
Figure 4. (a) The full spectrum and (b) C 1s of G@FeN-MP, GO@FeN-MP, ZnS-GO@FeN-MP-1and ZnS-GO@FeN-MP-2; (c) high-resolution Zn 2p and (d) S 2p spectra of ZnS-GO@FeN-MP-1and ZnS-GO@FeN-MP-2
表 1 实验合成材料统计表
Table 1. Statistical table of experimental synthetic materials:
Material Name Synthetic Raw Material Synthetic Method G@FeN-MP Fe、Fe3O4、Fe3N、C Microwave plasma
GO@FeN-MPG@FeN-MP CO2 plasma modification
ZnS-GO@FeN-MP-1GO@FeN-MP(20-50 mg)、ZnSO4·7H2O(1 mmoL,0.28756 g)、Na2S·9H2O
(1 mmoL,0.24018 g)、ethanediol (15 mL)
Solvothermal method
ZnS-GO@FeN-MP-2GO@FeN-MP(20-50 mg)、Na2S·9H2O(1 mmoL,0.24018 g)、Zn(CH3COO)2·2H2O(1 mmoL,0.21951 g)、ethanediol (15 mL)
Solvothermal method表 2 1 mg ZnS-GO@FeN-MP-1在450 nm下的吸光度值
Table 2. Absorbance value of 1 mg ZnS-GO@FeN-MP-1 at 450 nm
ZnS-GO@FeN-MP-1 Second Antibody -ZnS-GO@FeN-MP-1 Second Antibody 0.24 0.285 0.385 0.164 0.321 0.371 0.205 0.248 0.364 Average value 0.203 0.285 0.373 表 3 1 mg ZnS-GO@FeN-MP-2在450 nm下的吸光度值
Table 3. Absorbance value of 1 mg ZnS-GO@FeN-MP-2 at 450 nm
ZnS-GO@FeN-MP-2 Second Antibody-ZnS-GO@FeN-MP-2 Second Antibody 0.600 0.821 0.385 0.581 0.766 0.371 0.585 0.877 0.364 Average value 0.589 0.821 0.373 -
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