Preparation and characterization of a new pH-responsive hydrogel with bacteriostatic properties
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摘要: 为设计一种能够响应pH的、新型多功能创面敷料，使用季铵化壳聚糖(QCS)、接枝了磺胺嘧啶(SD)的氧化葡聚糖(Dex-CHO/SD)和妥布霉素(TOB)，通过席夫碱键和氢键反应形成DSTQ水凝胶。对水凝胶的形貌、降解性、抑菌性、细胞相容性、血液相容性等性能进行研究。结果表明：具有三维孔状结构的DSTQ水凝胶可以响应感染造成的pH降低，进而发生降解，释放药物；DSTQ水凝胶对铜绿假单胞菌的抑制率显著高于金黄色葡萄球菌；DSTQ水凝胶毒性低，生物安全性高，与L929细胞共培养3天后，细胞活力高于95%；DSTQ水凝胶具有良好的血液相容性，溶血率远小于5%；细胞在DSTQ水凝胶表面能够快速迁移，有利于伤口愈合；当DSTQ水凝胶负载生长因子后具有良好的抗炎效果。研究结果表明，DSTQ水凝胶可以作为新型创面敷料，有望应用于临床促伤口愈合。Abstract: To develop a novel wound dressing with multiple functionalities and pH-responsiveness, a combination of quaternary ammonium chitosan (QCS), oxidized dextran grafted with sulfadiazine (Dex-CHO/SD), and tobramycin(TOB) was employed to fabricate the DSTQ hydrogel through Schiff base bonding and hydrogen bonding. The hydrogel formation relied on Schiff base bonding and hydrogen bonding. The morphology, degradation, bacteriostatic, cytocompatibility, and hemocompatibility properties of the hydrogels were analyzed. The results indicated that the 3D pore structure of DSTQ hydrogel could respond to pH drops caused by infections and degrade, facilitating drug release. The inhibition rate of DSTQ hydrogel against Pseudomonas aeruginosa was significantly higher than that of Staphylococcus aureus. DSTQ hydrogel exhibited low toxicity, high biosafety, and cell viability above 95% after three days of co-culture with L929 cells. Additionally, it demonstrated excellent hemocompatibility, with a hemolysis rate below 5%. Furthermore, cells exhibited rapid migration on the material's surface, promoting wound healing. When loaded with growth factors, DSTQ hydrogel demonstrated good anti-inflammatory effects. These findings suggest that DSTQ hydrogel can be an effective wound-dressing option in clinical settings.
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Key words:
- hydrogel /
- wound healing /
- pH-responsive /
- antibacterial properties /
- wound dressing
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图 1 DSTQ水凝胶合成及作用机制图
Figure 1. Schematic diagram of the synthesis and action of DSTQ hydrogel
图 2 (a) Dex, Dex-CHO, Dex-CHO/SD的FT-IR图谱;(b) CS, QCS的FT-IR图谱; (c) DSTQ-1，DSTQ-2，DSTQ-3的FT-IR图谱Fig. 2(a) FT-IR spectra of Dex, Dex-CHO, Dex-CHO/SD; (b) FT-IR spectra of CS, QCS;(c) FT-IR spectra of DSTQ-1, DSTQ-2, DSTQ-3.
图 3 (a) DSTQ-1, (b) DSTQ-2和(c) DSTQ-3的SEM图像
Figure 3. SEM images of (a) DSTQ-1, (b) DSTQ-2, and (c) DSTQ-3
图 4 (a) DSTQ水凝胶黏附在皮肤、塑料及手套表面图片 (b) DSTQ水凝胶的黏附强度
Figure 4. (a) DSTQ hydrogels adhere to skin, plastics, and glove surfaces. (b) Adhesion strength of DSTQ hydrogels
图 5 DSTQ水凝胶在不同pH下的降解率(a) DSTQ-1(b) DSTQ-2(c) DSTQ-3
Figure 5. Degradation rate of DSTQ hydrogels (a) DSTQ-1 (b) DSTQ-2 (c) DSTQ-3
图 6 (a)DSTQ水凝胶对金黄色葡萄球菌和铜绿假单胞菌的抑菌图像及(b)金黄色葡萄球菌和(c)铜绿假单胞菌的定量统计结果。***代表有显著性差异(P＜0.001，n=3)
Figure 6. (a) Antibacterial images of Staphylococcus aureus and Pseudomonas aeruginosa and (b) Staphylococcus aureus and (c) Pseudomonas aeruginosa in DSTQ hydrogels. *** The asterisk indicates a significant difference (P<0.001，n=3)
图 7 DSTQ水凝胶对L929细胞活力的影响
Figure 7. Quantitative statistics of L929 cell viability by CCK-8.
图 8 (a)DSTQ水凝胶的溶血宏观图像及(b)定量结果
Figure 8. (a) Representative display pictures and (b) quantitative statistical data for hemolysis ratios of DSTQ hydrogels.
图 10 (a)HUVEC细胞迁移宏观图像及(b)定量统计结果。***代表有显著性差异(P＜0.001，n=3)
Figure 10. (a) Representative display pictures and (b) quantitative statistics of cell migration. *** The asterisk indicates a significant difference (P＜0.001，n=3)
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