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一种具有抑菌性的pH响应新型水凝胶的制备及性能

岳灿灿 赵鸿鹏 刘文森 许娜 张淑华 郗艳丽

岳灿灿, 赵鸿鹏, 刘文森, 等. 一种具有抑菌性的pH响应新型水凝胶的制备及性能[J]. 复合材料学报, 2024, 42(0): 1-11.
引用本文: 岳灿灿, 赵鸿鹏, 刘文森, 等. 一种具有抑菌性的pH响应新型水凝胶的制备及性能[J]. 复合材料学报, 2024, 42(0): 1-11.
YUE CanCan, ZHAO HongPeng, LIU WenSen, et al. Preparation and characterization of a new pH-responsive hydrogel with bacteriostatic properties[J]. Acta Materiae Compositae Sinica.
Citation: YUE CanCan, ZHAO HongPeng, LIU WenSen, et al. Preparation and characterization of a new pH-responsive hydrogel with bacteriostatic properties[J]. Acta Materiae Compositae Sinica.

一种具有抑菌性的pH响应新型水凝胶的制备及性能

基金项目: 吉林省科技厅科技发展计划项目 (20220204127YY);吉林省科技厅重点研发项目 (NOS.20220204035YY)
详细信息
    通讯作者:

    张淑华,博士,教授,硕士生导师,研究方向为生物材料与生物检测 E-mail: zhangsh@cust.edu.cn

    郗艳丽,博士,副教授,硕士生导师,研究方向为毒理学 E-mail: jilin2534126@163.com

  • 中图分类号: TB332

Preparation and characterization of a new pH-responsive hydrogel with bacteriostatic properties

Funds: Jilin Province Science and Technology Development Plan Project (20220204127YY); Jilin Province Science and Technology key research and Development Plan Project (NOS.20220204035YY)
  • 摘要: 为设计一种能够响应pH的、新型多功能创面敷料,使用季铵化壳聚糖(QCS)、接枝了磺胺嘧啶(SD)的氧化葡聚糖(Dex-CHO/SD)和妥布霉素(TOB),通过席夫碱键和氢键反应形成DSTQ水凝胶。对水凝胶的形貌、降解性、抑菌性、细胞相容性、血液相容性等性能进行研究。结果表明:具有三维孔状结构的DSTQ水凝胶可以响应感染造成的pH降低,进而发生降解,释放药物;DSTQ水凝胶对铜绿假单胞菌的抑制率显著高于金黄色葡萄球菌;DSTQ水凝胶毒性低,生物安全性高,与L929细胞共培养3天后,细胞活力高于95%;DSTQ水凝胶具有良好的血液相容性,溶血率远小于5%;细胞在DSTQ水凝胶表面能够快速迁移,有利于伤口愈合;当DSTQ水凝胶负载生长因子后具有良好的抗炎效果。研究结果表明,DSTQ水凝胶可以作为新型创面敷料,有望应用于临床促伤口愈合。

     

  • 图  1  DSTQ水凝胶合成及作用机制图

    Figure  1.  Schematic diagram of the synthesis and action of DSTQ hydrogel

    图  2  (a) Dex, Dex-CHO, Dex-CHO/SD的FT-IR图谱;(b) CS, QCS的FT-IR图谱; (c) DSTQ-1,DSTQ-2,DSTQ-3的FT-IR图谱Fig. 2(a) FT-IR spectra of Dex, Dex-CHO, Dex-CHO/SD; (b) FT-IR spectra of CS, QCS;(c) FT-IR spectra of DSTQ-1, DSTQ-2, DSTQ-3.

    图  3  (a) DSTQ-1, (b) DSTQ-2和(c) DSTQ-3的SEM图像

    Figure  3.  SEM images of (a) DSTQ-1, (b) DSTQ-2, and (c) DSTQ-3

    图  4  (a) DSTQ水凝胶黏附在皮肤、塑料及手套表面图片 (b) DSTQ水凝胶的黏附强度

    Figure  4.  (a) DSTQ hydrogels adhere to skin, plastics, and glove surfaces. (b) Adhesion strength of DSTQ hydrogels

    图  5  DSTQ水凝胶在不同pH下的降解率(a) DSTQ-1(b) DSTQ-2(c) DSTQ-3

    Figure  5.  Degradation rate of DSTQ hydrogels (a) DSTQ-1 (b) DSTQ-2 (c) DSTQ-3

    图  6  (a)DSTQ水凝胶对金黄色葡萄球菌和铜绿假单胞菌的抑菌图像及(b)金黄色葡萄球菌和(c)铜绿假单胞菌的定量统计结果。***代表有显著性差异(P<0.001,n=3)

    Figure  6.  (a) Antibacterial images of Staphylococcus aureus and Pseudomonas aeruginosa and (b) Staphylococcus aureus and (c) Pseudomonas aeruginosa in DSTQ hydrogels. *** The asterisk indicates a significant difference (P<0.001,n=3)

    图  7  DSTQ水凝胶对L929细胞活力的影响

    Figure  7.  Quantitative statistics of L929 cell viability by CCK-8.

    图  8  (a)DSTQ水凝胶的溶血宏观图像及(b)定量结果

    Figure  8.  (a) Representative display pictures and (b) quantitative statistical data for hemolysis ratios of DSTQ hydrogels.

    图  9  DSTQ水凝胶的细胞黏附率

    Figure  9.  Cell adhesion capacity of DSTQ hydrogels.

    图  10  (a)HUVEC细胞迁移宏观图像及(b)定量统计结果。***代表有显著性差异(P<0.001,n=3)

    Figure  10.  (a) Representative display pictures and (b) quantitative statistics of cell migration. *** The asterisk indicates a significant difference (P<0.001,n=3)

    图  11  各组细胞炎症因子(a) IL-1β,(b) IL-6和(c) TNF-α的表达水平。***代表有显著性差异(P<0.001,n=3)

    Figure  11.  Expression levels of inflammatory cytokines (a) IL-1β, (b) IL-6, and (c) TNF-α in each group. *** The asterisk indicates a significant difference(P<0.001,n=3)

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出版历程
  • 收稿日期:  2024-01-16
  • 修回日期:  2024-04-03
  • 录用日期:  2024-04-14
  • 网络出版日期:  2024-05-20

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