Biomechanical properties of a customizable thermoplastic polyurethane/ polycaprolactone blended esophageal stent fabricated by 3D printing
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摘要: 食管支架在食管疾病的临床治疗中有广泛的应用。但商业食管支架尺寸固定,无法根据患者食管的几何形状进行个性化定制。通过熔融共混结合3D打印技术的方式制备了一种热塑性聚氨酯(TPU)/聚己内酯(PCL)共混物食管支架,并基于对TPU/PCL组分比例的调节,实现了对支架力学性能的调控。通过差示扫描量热、热重分析、力学试验、体外降解试验和细胞毒性检测研究了TPU/PCL共混物的理化特性及支架的各项关键指标。结果表明,TPU/PCL共混物具有良好的材料相容性和力学性能,且细胞活性为72.9%,对细胞无潜在毒性。经过为期8周的体外降解试验,支架的重量损失均小于2%,且力学性能无明显变化。3D打印TPU/PCL共混物支架优越的力学和生物学性能证明了其在食管狭窄的临床个性化治疗中具有潜在应用前景。Abstract: Esophageal stents were widely used in the clinical therapy of esophageal diseases. However, commercial esophageal stents had fixed sizes and cannot be personalized according to esophageal geometry of patient. A thermoplastic polyurethane (TPU)/polycaprolactone (PCL) blend esophageal stent was prepared by melt blending combined with 3D printing technology. Based on the adjustment of TPU/PCL component ratio, the mechanical properties of the stent were regulated. The physicochemical properties of TPU/PCL blends and the key parameters of the stents were investigated by differential scanning calorimetry, thermogravimetric analysis, mechanical tests, in vitro degradation tests and cytotoxicity assays. The results show that the TPU/PCL blends exhibit favorable compatibility and mechanical properties, and the cellular activity is 72.9% with no potential toxicity to cells. The weight of the stents is less than 2%, and the mechanical properties do not change significantly after 8-week in vitro degradation test. The superior mechanical and biologic features of 3D printed TPU/PCL blended stent demonstrate the potential application in the clinical personalized treatment of esophageal stenosis.
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Key words:
- 3D printing /
- Esophageal stent /
- Mechanical property /
- TPU/PCL /
- Biocompatibility
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表 1 不同组分比TPU/PCL共混物的打印参数
Table 1. Printing parameters of TPU/PCL blends with different component ratios
Sample Nozzle
temperature/℃Build plate
temperature /℃TPU 210 50 80TPU/20PCL 210 35 70TPU/30PCL 210 35 60TPU/40PCL 210 35 PCL 100 35 Notes: Printing speed: 20 mm/s. Layer height: 0.1 mm. 表 2 不同组分比TPU/PCL共混物的弯曲力学性能
Table 2. Flexural mechanical properties of TPU/PCL blends with different component ratios
Sample Flexural modulus/MPa Flexural strength/MPa Maximal force/N TPU 9.0±0.27 0.8±0.01 1.2±0.03 80TPU/20PCL 23.7±0.47 1.6±0.05 3.1±0.22 70TPU/30PCL 42.0±0.00 2.3±0.04 3.8±0.06 60TPU/40PCL 62.0±4.08 3.0±0.04 5.0±0.07 PCL 416.7±4.72 13.9±0.03 23.1±0.05 -
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