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基于PVA/BNC复合材料的人工耳廓的制备与性能

黄丽娜 陈琳 洪枫

黄丽娜, 陈琳, 洪枫. 基于PVA/BNC复合材料的人工耳廓的制备与性能[J]. 复合材料学报, 2024, 42(0): 1-10.
引用本文: 黄丽娜, 陈琳, 洪枫. 基于PVA/BNC复合材料的人工耳廓的制备与性能[J]. 复合材料学报, 2024, 42(0): 1-10.
HUANG Lina, CHEN Lin, HONG Feng. Preparation and properties of artificial auricles based on PVA/BNC composites[J]. Acta Materiae Compositae Sinica.
Citation: HUANG Lina, CHEN Lin, HONG Feng. Preparation and properties of artificial auricles based on PVA/BNC composites[J]. Acta Materiae Compositae Sinica.

基于PVA/BNC复合材料的人工耳廓的制备与性能

详细信息
  • 中图分类号: R318.08;TB332

Preparation and properties of artificial auricles based on PVA/BNC composites

  • 摘要: 耳廓再造的关键是实现植入材料与天然组织间的生物力学适配,然而迄今尚未找到一种理想的耳廓替代物。本研究将细菌纳米纤维素(Bacterial nanocellulose, BNC)匀浆加至不同浓度的聚乙烯醇(Polyvinyl alcohol, PVA)水溶液中,通过冻融法使两者物理交联形成兼具高弹柔韧性和高力学强度的PVA/BNC复合材料,并对其理化性能和细胞相容性进行表征。结果表明:该材料具有高吸水性、低溶胀比,较高的韧性、弹性和缝合强度等特点,最大压缩模量可达到6.98 ± 0.49 MPa,与天然耳廓组织的生物力学相适配。最大缝合强度可达7.06 ± 0.33 N,完全满足临床缝合需求。BNC的加入促进了细胞在材料表面的粘附、生长和增殖,使PVA/BNC复合材料具有更高的细胞密度和活力,是一种很有潜力的人工耳廓材料。

     

  • 图  1  PVA、PVA/BNC材料的宏观、微观形貌

    Figure  1.  Macroscopic and microscopic morphologies of PVA and PVA/BNC materials (a) Macroscopic morphology of PVA and PVA/BNC materials with different concentrations; (b) Diagram of an auricular sample; (c) Scanning electron microscope microscopic topography of materials

    图  2  材料的基本理化性质

    (a)不同浓度PVA、PVA/BNC材料的红外光谱图;(b)含水率;(c)吸水率;(d)饱和溶胀率;(e)X射线衍射图谱

    Figure  2.  Basic physical and chemical properties of materials

    (a) Fourier transform infrared spectra of PVA and PVA/BNC materials with different concentrations;(b) Moisture content; (c) Water absorption rate;(d) Saturated swelling rate; (e) X-ray diffraction pattern

    图  3  PVA和PVA/BNC材料的压缩力学性能与缝合强度

    (a)不同浓度PVA、PVA/BNC材料的压缩强度;(b)压缩模量;(c)压缩应力-应变曲线;(d)16%PVA和PVA/BNC循环压缩应力-应变曲线;(e)循环压缩能量耗散与能量损耗系数;(f)缝合强度

    Figure  3.  Compressive mechanical properties and suture strength of PVA and PVA/BNC materials

    (a) Compressive strength of PVA and PVA/BNC materials with different concentrations; (b) Compressive modulus; (c) Compressive stress-strain curves; (d) 16% PVA and PVA/BNC cyclic compressive stress-strain curves; (e) Cyclic compression energy dissipation and energy dissipation coefficient;(f) Suture retention

    图  4  PVA、PVA/BNC材料的拉伸力学性能

    (a)各PVA、PVA/BNC材料的拉伸强度;(b)拉伸模量;(c)断裂伸长率;(d)拉伸应力-应变曲线;(e)16%PVA和PVA/BNC循环拉伸应力-应变曲线;(f)循环拉伸韧性与能量损耗系数

    Figure  4.  Tensile mechanical properties of PVA and PVA/BNC materials

    (a) Tensile strength of PVA and PVA/BNC materials; (b) Tensile modulus; (c) Elongation at break; (d) Tensile stress-strain curve; (e) 16% PVA and PVA/BNC cyclic tensile stress-strain curves; (f) Cyclic tensile toughness and energy dissipation coefficient

    图  5  小鼠成纤维细胞的相容性

    (a) CCK-8实验结果;(b)浸提液培养的细胞存活率;(c)浸提液培养L929细胞1、2、3天后细胞荧光图;(d)电镜显示细胞在材料上生长1、3、5天后的形态图

    Figure  5.  Cytocompatibility of mouse fibroblasts

    (a) CCK-8 experimental results; (b) Cell viability of extract culture; (c) Cell fluorescence pattern of L929 cells after culture in extract for 1, 2 and 3 days; (d) Electron microscopy showing morphology of cells after 1, 3, and 5 days of growth on the material

    表  1  孔隙率与比表面积测试结果

    Table  1.   Porosity and specific surface area test results

    Sample Skeletal density/(g·cm−3) Apparent density/(g·cm−3) Porosity/% Specific surface area/(m2·g−1)
    BNC
    16%PVA
    16%PVA/BNC
    1.056 ± 0.017
    1.219 ± 0.006
    1.207 ± 0.005
    0.009
    0.909
    0.455
    99.17
    25.47
    62.27
    58.98
    24.96
    26.48
    Notes: BNC—Bacterial nanocellulose; PVA—Polyvinyl alcohol
    下载: 导出CSV

    表  2  材料的结晶度

    Table  2.   Crystallinity of materials

    SampleCrystallinity/%
    BNC44.73 ± 5.07
    8%PVA34.08 ± 4.36
    12%PVA34.66 ± 9.87
    16%PVA35.80 ± 8.60
    20%PVA36.15 ± 6.28
    8%PVA/BNC37.38 ± 7.52
    12%PVA/BNC38.39 ± 7.88
    16%PVA/BNC45.68 ± 3.94
    20%PVA/BNC40.07 ± 1.47
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-12-19
  • 录用日期:  2024-01-26
  • 网络出版日期:  2024-02-24

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