Abstract: The oxidase catalyst in the third-generation glucose sensors can be easily affected by temperature, humidity, and pH. As a result, developing some catalysts with low cost and high sensitivity has a broad application prospect. Metal-organic frameworks (MOFs) have attracted much attention because of their high mass transfer rate, adjustable porosity and strong electron transfer ability. The screen-printing technique has been used to fabricate commercial electrodes due to its low cost and batch preparation. Here, Co-MOFs were successfully synthesized by a simple and economical method at room temperature and coated on commercial strip silver-carbon electrode by screen-printing technique. As a biosensor catalyst, Co-MOFs nanomaterials exhibit high electrocatalytic activity for glucose. The results showed that the sensitivity of Co-MOFs-based strip electrode for glucose detection was 1393 nA·L/(mmol·cm²). The detection limit was 0.58 μmol/L (S/N=3) and the linear range was 0.1-0.5 mmol/L. This work has certain guiding significance for the design of multi-function electrode in glucose sensor.