单宁酸包裹的阿维菌素/介孔SiO<sub>2</sub>纳米载药系统的构建与性能

徐鹏; 戴伟; 曹蓉; 石伟山; 邢刚; 王钊贵; 王莎莎; 李群; 游朝群; 郝德君

doi:10.13801/j.cnki.fhclxb.20230828.002

单宁酸包裹的阿维菌素/介孔SiO₂纳米载药系统的构建与性能

doi: 10.13801/j.cnki.fhclxb.20230828.002

徐鹏^1, ,,
戴伟¹,
曹蓉²,
石伟山³,
邢刚³,
王钊贵⁴,
王莎莎¹,
李群¹,
游朝群¹,
郝德君⁴

1.
南京林业大学　化学工程学院，南京 210037
2.
南京市六合区农业技术推广中心　森林植物检疫站，南京 211500
3.
江苏艾津作物科技集团有限公司，南京 211511
4.
南京林业大学　林学院，南京 210037

基金项目: 江苏省林业科技创新与推广项目(LYKJ-南京[2022]02)

详细信息

通讯作者:
徐鹏，博士，副教授，硕士生导师，研究方向为纳米农药开发与应用　E-mail: xupeng@njfu.edu.cn

中图分类号: TB332
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出版历程
- 收稿日期: 2023-06-05
- 修回日期: 2023-07-22
- 录用日期: 2023-07-29
- 网络出版日期: 2023-08-29
- 刊出日期: 2024-03-01

Preparation and properties of tannic acid coated abamectin/mesoporous silica nano-pesticide delivery system

1.
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
2.
Forest Plant Quarantine Station, Agricultural Technology Promotion Center of Luhe District of Nanjing City, Nanjing 211500, China
3.
Jiangsu Aijin Crop Technology Group CO., LTD., Nanjing 211511, China
4.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Funds: Jiangsu Forestry Science and Technology Innovation and Promotion Project (LYKJ-Nanjing[2022]02)

摘要

摘要: 增加农药在靶标作物叶面的滞留时间对提高农药利用率和降低农药对环境的影响具有重要意义。本文以阿维菌素(Aba)为模型药物，以单宁酸(TA)包裹的介孔SiO₂纳米颗粒为载体材料构建纳米农药载药系统，在对纳米农药载药系统结构和形貌进行表征的基础上，通过模拟释放实验、植物叶面接触角和滞留量的比较及抗紫外光解实验深入研究纳米载药系统的释放性能和叶面粘附性能。研究发现，单宁酸包覆的阿维菌素/介孔SiO₂纳米载药系统(Aba/MSNs@TA)的载药量达到23.50%，单宁酸的包覆明显提高了载药系统在绿萝、玉米和马尾松叶面上的润湿性，叶面滞留量相较于Aba/MSNs提高了23.4%。Aba/MSNs@TA表现出明显的pH响应性释放性能，较低的pH值环境加速了Aba的释放速度。此外，单宁酸的包覆进一步提高了载药系统中药物的抗紫外光解性能。
- 阿维菌素 /
- 粘附性 /
- 介孔SiO₂ /
- 刺激响应 /
- 抗光降解 /
- 单宁酸 /
- 农药载药系统
Abstract: It is important to increase the retention time of pesticides on the leaf surface of target crops for improving pesticide utilization and reducing the impact of pesticides on the environment. In this paper, we used abamectin (Aba) as a model pesticide and mesoporous silica nanoparticles coated with tannic acid (TA) as a carrier material to construct a nano-pesticide delivery system. Based on the structural and morphological characterization of the nano-pesticide delivery system, we investigated the release performance and foliar adhesion of the nano-pesticide delivery system through simulated release experiments, comparison of contact angle and retention amount on plant foliage and UV photolysis resistance experiments. It is found that tannic acid-coated abamectin-loaded mesoporous silica nanospheres (Aba/MSNs@TA) significantly improve drug wettability on the foliage of Epipremnum aureum, corn and masson pine, and foliar retention is also improved compared to Aba/MSNs. Aba/MSNs@TA exhibits significant pH-responsive release performance, with lower pH environments accelerating the release rate of Aba. In addition, the coating of tannic acid further improves the UV photolytic resistance of the drug in the drug-loaded system.
- abamectin /
- adhesion /
- mesoporous SiO₂ /
- stimulus response /
- anti-photodegradation /
- tannic acid /
- pesticide delivery system

HTML全文

图 1 ((a), (b)) 负载阿维菌素的介孔 SiO₂纳米颗粒(Aba/MSNs)的TEM图像；(c) 单宁酸(TA)包覆的阿维菌素/介孔SiO₂纳米载药系统(Aba/MSNs@TA)的TEM图像；(d) Aba/MSNs@TA和Aba/MSNs的实物图

Figure 1. ((a), (b)) TEM images of abamectin-loaded mesoporous silica nanospheres (Aba/MSNs); (c) Tannic acid (TA)-coated abamectin/mesoporous silica nanospheres (Aba/MSNs@TA); (d) Photo of Aba/MSNs@TA and Aba/MSNs

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图 2 Aba、MSNs、Aba/MSNs和Aba/MSNs@TA的FTIR图谱 (a)；Aba/MSNs和Aba/MSNs@TA的吸脱附等温线(b)、DTG曲线(c)、TGA曲线(d)

Figure 2. FTIR spectra of Aba, MSNs, Aba/MSNs and Aba/MSNs@TA (a) ; N₂ adsoprtion-desorption (b), DTG curves (c) and TGA curves (d) of Aba/MSNs and Aba/MSNs@TA

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图 3 (a) Aba的紫外光谱图；(b) Aba-甲醇标准曲线

Figure 3. (a) Ultraviolet spectra of Aba; (b) Aba-methanol standard curve

R²—Linear correlation coefficient

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图 4 在乙醇/水(体积比30/70)混合液中，Aba/MSNs@TA和Aba/MSNs中Aba的释放曲线

Figure 4. Release curves of Aba from Aba/MSNs@TA and Aba/MSNs in ethanol/water (volume ratio 30/70) mixtures

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图 5 不同pH的磷酸盐缓冲溶液(PBS)条件下，Aba/MSNs@TA中Aba的释放曲线

Figure 5. Release curves of Aba from Aba/MSNs@TA under different pH conditions of phosphate buffer solution (PBS)

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图 6 Ritger-Peppas方程(a)、零级方程(b)、一级方程(c)和Higuchi方程(d)拟合曲线

Figure 6. Fitting plots using the Ritger-Peppas equation (a), the Zero-level equation (b), the First-level equation (c), and the Higuchi equation (d)

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图 7 不同浓度的Aba/MSNs@TA和Aba/MSNs在叶面上的滞留量

Figure 7. Retention of Aba/MSNs@TA and Aba/MSNs on leaves at different concentrations

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图 8 Aba、MSNs、Aba/MSNs和Aba/MSNs@TA在不同叶面上的接触角：(a) 绿萝；(b) 玉米；(c) 马尾松

Figure 8. Contact angle of Aba, MSNs, Aba/MSNs and Aba/MSNs@TA on different leaves: (a) Epipremnum aureum; (b) Corn; (c) Masson pine

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图 9 Aba/MSNs和Aba/MSNs@TA在绿萝叶面荧光成像

Figure 9. Fluorescence images of Aba/MSNs and Aba/MSNs@TA on the surface of Epipremnum aureum foliage

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图 10 Aba/MSNs和Aba/MSNs@TA在不同紫外照射时间下的Aba剩余率

Figure 10. Retention rate of Aba/MSNs and Aba/MSNs@TA under different UV irraditation time

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表 1 文献中不同载药系统载药量的对比

Table 1. Comparison of drug loading capacities of different drug loading systems in literature

Carrier material	Pesticides	Loading rate/%	Ref.
MSNs-chitosan@prochloraz nanoparticles	Prochloraz	25.40	[20]
Avermectin@MSNs-SS-starch nanoparticles	Avermectin	9.30	[21]
DMM@HMS-SS-COS	Dimethomorph	24.36	[22]
Py@F-DSH-MSNs	Pyraclostrobin	28.50	[23]
CHT@MSNs-β-glucans	Chlorothalonil	24.99	[24]
Aba/MSNs@TA	Abamectin	23.50	This study
Notes: MSNs—Mesoporous silica nanoparticles; SS—Disulfide bond; DMM—Dimethomorph; HMS—Hollow mesoporous silica; COS—Chitosan oligosaccharide; Py—Pyraclostrobin; F-DSH-MSNs—Fluorophore-free luminescent double-shelled hollow MSNs; CHT—Chlorothalonil; MSNs-β-glucans—β-glucans attached MSNs.

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表 2 通过拟合几个动力学方程计算Aba/MSNs@TA释放Aba的参数

Table 2. Parameters of Aba released from Aba/MSNs@TA by fitting several kinetic equations

Model	pH value	a	n	r²
Zero-order	5	0.469	—	0.825
	7	0.329	—	0.878
	9	0.132	—	0.831
First-order	5	86.801	—	0.998
	7	61.858	—	0.996
	9	25.843	—	0.956
Higuchi	5	7.415	—	0.961
	7	5.094	—	0.982
	9	2.088	—	0.966
Ritger-Peppas	5	7.537	0.488	0.960
	7	4.235	0.526	0.978
	9	3.764	0.396	0.982
Notes: a—Release rate constant; n—Release characteristic index; r²—Regression coefficient.

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