Abstract: The silk graft polyacrylonitrile-polyacrylamide (SF-g-P(AN-co-AM)) composite filtration membrane was produced using zinc chloride aqueous solution as the solvent and using water as the coagulation bath. The SF-g-P(AN-co-AM) composite filtration membrane was characterized by SEM, TG analysis and water contact angle. The mechanical properties, separation performance and anti-fouling performance of the SF-g-P(AN-co-AM) membrane were tested. The results show that the fracture strength of the SF-g-P(AN-co-AM) membrane increases with the increasing of the acrylamide content, but the flux decreaseds. When 0.5 g of acrylamide is used, the fracture strength of the SF-g-P(AN-co-AM) membrane reaches 1.4 MPa. The SF-g-P(AN-co-AM) membrane has good separation performance, when the water flux is 35 L/(m²h) under 0.1 MPa pressure and the rejection rate of 0.03 g/L Congo red (M_w=696.68) solution reaches 99%. The SF-g-P(AN-co-AM) membrane exhibits good anti-fouling performance, and the flux recovery rate is 94.4% after three cycles of pure water and bovine serum albumin solution filtration without cleaning.