Abstract: A method was investigated for preparing bioactive n-HA (nano-hydroxyapatite) and PA66 (Polyamide 66) nano-composite as the scaffold for the hard tissue repair using integrating composite with mold and pore. SEM, XRD, IR and burning test were used to analyze the physical and chemical properties of the scaffold. The results indicate that n-HA particles disperse with nano scale in the composite; there are hydrogen bonding and ligand bonding between HA and PA; the scaffold has three-dimensional pored structure with 450μm macropores and 0.5~50μm micropores. Animal testing shows that there are good histological compatibility and high bioactivity between the porous scaffold and bone. The scaffold is a kind of excellent carriers for bone repair because it produces bone-bonding with host and implant and its different pore sizes are beneficial to the growth of bone tissue, blood vessels and osteocytes.