淀粉/PVA膜在血浆模拟液及唾液模拟液中的体外降解行为及相关体外生物相容性评价

In vitro degradation behaviour of starch/PVA films in simulated blood and simulated saliva fluid and in vitro biocompatibility evaluation

摘要: 通过熔融浇注法制备出一系列厚度在0.05 mm～0.10 mm的淀粉/聚乙烯醇（PVA）（SP）薄膜。研究了薄膜在血浆（SBF）及唾液模拟液（SSF）中的降解行为，分析了降解过程中力学性能、失重率、溶胀度、热性能以及表面形态的变化。研究结果表明，膜在30天的降解过程中能够维持良好的尺寸稳定性和一定的力学强度。通过细胞毒性、细胞贴壁及溶血试验表征了膜的体外生物相容性。结果表明，SP膜具有良好的细胞和血液相容性。所有测试结果证明，SP薄膜是一种应用于诱导组织再生薄膜的潜在材料。

Abstract: A series of starch/PVA (SP) films with the thickness of 0.05 mm～0.10 mm were casted by the solvent method. The degradation behaviors of the SP films in simulated blood fluid (SBF) and simulated saliva fluid (SSF) were studied. Changes of their mechanical properties, mass loss, swelling degree, thermal properties and morphology of surface during degradation were tested. It is found that the films can keep the shape stability and certain mechanical properties during 30 days degradation. The biocompatibility of the SP film was investigated by cytotoxicity, hemolytic and cell culture tests. The results show that the SP films possess excellent cell and blood compatibility. All the results demonstrate that the SP films can be potentially applied as guide tissue regeneration films.