Abstract: Nano-hydroxyapatite/polyamide 66(n-2HA/PA66) and Ag loaded hydroxyapatite/titania/polyamide 66 (Ag-HA-TiO₂/PA66) composite membranes for guided bone regeneration (GBR) were prepared by the phase inversion method. Biocompatibility of the two kinds membranes was assessed via 32(4，52dimethylthiazol222yl)22，52 diphenyl-tetrazolium-bromide assay (MTT) and flow cytometry (FCM) test. The fabricated membranes have asymmetric porous structure. SEM analysis reveals that pores less than 10μm and pores with a size ranging from 30μm to 200μm distribute in the micropore layer and the spongy structure layer，respectively. MTT and FCM results indicate that the two kinds membranes have good cell affinity，while Ag-HA-TiO₂/PA66 membrane can promote cell apopotosis to some extent. The structure and biocompatibility of the fabricated membranes can meet the requirements of GBR.