Abstract: In recent years, the application of conductive polymer materials in the field of flexible wearable electronics has been increasingly prominent. Compared with film materials, fibers have inherent advantages in flexibility, weavability, etc. Wet spinning technology is the main means of continuous preparation of conductive fibers, and the PEDOT:PSS-based fibers have the advantages of flexibility, high electrical conductivity, large specific surface area, spinnability, and so on. However, the rigidity of the PEDOT main chain prevents the simultaneous satisfaction of fiber stretchability and conductivity, limiting its application in the field of flexible wearable electronics. Therefore, research on wet spinning to prepare high-performance conductive fibers has become a current focus and challenge. By optimizing the key steps in the wet spinning process, the comprehensive performance of fibers can be effectively enhanced, thereby providing new possibilities for the application of conductive fibers in the future field of flexible electronics. In this review, we summarize the current preparation strategy of wet spinning PEDOT:PSS-based fibers, including the three key steps of spinning solution design, coagulation bath regulation and post-treatment optimization, analyze the applications and challenges of PEDOT:PSS-based fibers in the field of flexible electronic devices, and provide prospects for the performance and development direction of PEDOT:PSS-based fibers in next-generation fiber-based flexible electronic devices.