Abstract: Ternary mixture solution of Zn(Ac)₂/polyethylene glycol 600 (PEG600)/H₂O was used as precursor, Zn(Ac)₂ was hydrolyzed via two-step heating processes at temperature range of 100-200 ℃ and finally ZnO seeds formed on hole wall of SiO₂ by calcination at high temperature. Zinc-ammonia complex solution was used as zinc source, at 90 ℃ it was thermally decomposited and the resulted Zn(OH)₂ was deposited in the channels. ZnO nanowires were in-situ prepared through hydrothermal synthesis at 100 ℃. The compositions of ternary precursor were changed to control the size and distribution of ZnO seeds which could further control the morphology of ZnO nanowires. And ZnO nanowires with diameter of 15-20 nm were eventually obtained, which were uniformly dispersed in the 3D macropores as random coil. XRD and Raman spectroscopy confirm that the nanowire has hexagonal wurtzite crystal structure. The adsorption properties of ZnO nanowire/macroporous SiO₂ composites were investigated by testing its immobilizing ability against the porcine pancreatic lipase. The experimental results show that the adsorption capacity of composites is 5-6 times than that of macroporous SiO₂ materials. The maximum of adsorption capacity is up to 286.8 mg·g^-1 and the highest enzyme activity is 425.5 U·g^-1. Both adsorption capacity and the immobilizing ability against lipase remain almost unchanged when the samples are soaked in buffer solution for 48 h.