Abstract: Tourmaline/chitosan(Tur/CS) composite fibers were prepared with Chitosan as the matrix and tourmaline as the dispersed phase by solution spinning, and characterized by optical microscope, SEM and FTIR. MG63 cells were co-cultured with Tur/CS composite fibers to assess the cytocompatibility. The results show that the tourmaline particles were wrapped by chitosan and fully dispersed in the composite fiber. There were no bare tourmaline particles on the surface. Cytocompatibility test using MG63 revealed positive cell viability and proliferation over a period in vitro. And materials appeared no apparent toxicity. These results indicate that the material can be a kind of good wound repair dressing.