Abstract: In order to improve lower mechanical property, transparency, swelling ratio and biocompatibility of traditional chemical-cross-linked hydrogel, the temperature-sensitive and biocompatible nanocomposite hydrogel was prepared by in-situ free-radical polymerization by adopting lithium magnesium silicate hydrate (LMSH) as physical cross-linker, galactosylated acrylate (GAC) as biocompatible monomer, and isopropylacrylamide(NIPAM) as functional monomer. The results show that LMSH platelets were completely exfoliated and act as cross-linker. In comparison with traditional chemical cross-linked hydrogel, the resulting physical cross-linked nanocomposite hydrogel exhibits high swelling ratio, good temperature-sensitivity, and excellent impulse responsibility, but poor L929 cell viability on the surface of nanocomposite hydrogel. The volume phase transition temperature (VPTT) has little change by using LMSH as cross-linker and hydrophilic GAC, keeping still at 33℃.