Abstract: N-isopropylacrylamide(NIPAM) and acrylic acid (AA)copolymer microspheres with various compositions were prepared by a reverse suspension emulsion technique. The microspheres were employed as microreactors for the synthesis of fluorescence composite microspheres by two different methods. In this way, several uniform of P(NIPAM-co-AA)-Alq3 fluorescence composite microspheres were prepared. The surface functionalized P(NIPAM-co-AA)-Alq3-SiO₂ fluorescence composite microspheres was prepared by decorating the surface with the tetraethoxy-silicone(TEOS). The fluorescence composite microspheres were characterized by SEM, IR, FM and XRD. The results indicate uniform particle size fluorescent composite microspheres are obtained with high efficiency fluorescent light and high specific surface area. It might be expected that this functional material can be applied for high-throughput screening assays and recognition of biomacromolecules in biological medical fields.