Abstract: Keratin extracted from animal hair has biocompatibility and biodegradability, and it contains amino acid sequences with cell adhesion sites. Based on these excellent biological properties, keratin is considered a high-quality renewable protein resource applicable in biomedicine and holds significant value and potential in the application of tissue-engineered skin. Due to the evident differences between artificial skin tissues and autologous skin tissues, the perfect repair and reconstruction of skin wounds have always been a difficult problem and challenge in the biomedical field. The emergence of tissue-engineered skin provides a new approach for the repair of large-area wounds. This review mainly introduces keratin and its extraction process, the forms of keratin-based biomaterials, and discusses the functional mechanisms of keratin-based materials in skin tissue regeneration by regulating cell capabilities and wound healing effects, aiming to provide theoretical references for keratin in the field of tissue-engineered skin research.