Abstract: The natural enzymes in glucose sensors are susceptible to environmental factors such as temperature, humidity and pH. The oxidase catalyst in glucose sensors can be easily affected by temperature, humidity, and pH. As a result, developing some catalysts with low cost and high sensitivity has a broad application prospect. Metal-organic frameworks (MOFs) have attracted much attention because of their high mass transfer rate, adjustable porosity and strong electron transfer ability. The screen-printing technique has been used to fabricate commercial electrodes due to its low cost and batch preparation. Here, Co-MOFs were successfully synthesized by a simple and economical method at room temperature and coated on commercial strip silver-carbon electrode by screen-printing technique. As a biosensor catalyst, Co-MOFs nanomaterials exhibit high electrocatalytic activity for glucose. The results showed that the sensitivity of Co-MOFs-based strip electrode for glucose detection was 1393 nA·L/(mmol·cm²). The detection limit was 0.58 μmol/L (S/N=3) and the linear range was 0.1-0.5 mmol/L. This work has certain guiding significance for the design of multi-function electrode in glucose sensor.