Abstract: The antibacterial and conductive hydrogels based on silver nanoparticles (AgNPs) have important applications in wearable devices, electronic skin, biosensors, and other areas, and their green manufacturing is currently the focus of much research. Nanocellulose (CNF) has attracted more attention relating to the preparation and application of the smart hydrogels due to its unique physico-chemical properties. When AgNPs are combined with CNF and applied to hydrogels, it is expected to lead to fabrication of antibacterial hydrogels with good mechanical properties, which has guiding significance for the application of hydrogels in the area of intelligent wearable systems. In the present study, Ag-CNF composites were in situ synthesized using 2, 2, 6, 6-tetramethylpiperidoxyl (TEMPO)-oxidized CNF (TOCNF) as a composite substrate and AgNO₃ as a source of silver. Ag-CNF and tannic acid (TA) were introduced to polyacrylamide (PAM) hydrogels as functional additives to prepare the Ag-CNF/PAM hydrogel with good tensile properties, adhesion, antibacterial properties, and UV-shielding properties. The Ag-CNF/PAM hydrogels were then packaged into a strain-responsive sensor. The electrical and sensing properties were studied. Ag-CNF/PAM hydrogels can maintain stable and repeated electrical output under 100% strain cycle and can also be used for wrist motion and head motion detection, which has potential for use as a strain-responsive sensor.