Abstract: In this paper, hollow mesoporous SiO₂ nanocarriers (HMSNs-69-FITC) with fluorescence labeling were prepared by self-template method using polyacrylic acid (PAA), tetraethyl orthosilicate (TEOS) and silane coupling agent Si-69 as the main raw materials and fluorescein isothiocyanate (FITC) as the fluorescent agent. The structure and particle size of nanocarriers were determined by FTIR, DLS, BET, Raman and TEM, their reduction-sensitive properties were characterized by ultraviolet spectrophotometer and TEM, and sorafenib (SOR) was loaded with solvent volatilization, and calculate its load efficiency. The results show that the amount of regulating PAA can achieve controllable particle size of HMSNs in the range of 28-380 nm, among which, HMSNs with 0.024 g/mL PAA and an average particle size of 100 nm have excellent stability performance, HMSNs-69-FITC has a loading efficiency of 280.0 μg/mg for SOR, and in phosphate buffered saline (PBS) solution containing 0.0083 g/mL dithiothreitol (DTT), the cumulative release rate of 48 h is about 82.4%. In the absence of DTT, the cumulative release rate at 48 h is about 25.1%, which has significant disulfide bond reduction sensitivity. This work helps advance research in the field of particle size controllable and reduction-sensitive SiO₂ nanocarriers.