Abstract: Implantation/interventional surgery is the main treatment for cardiovascular diseases. Direct contact with blood during device implantation and damage to the implantation site can cause complications such as thrombosis and inflammation. Surface modification of materials is one of the main methods to improve the biocompatibility of materials. In this study, the dendrimer polyamide amine (PAMAM) was immobilized on the surface of alkaline activated Ti, and pyrroloquinoline quinone (PQQ), an anti-inflammatory and anti-oxidative molecule, was grafted on the amino group of PAMAM to construct the PAMAM-PQQ functional layer. The effects of different concentrations of PQQ on the surface biocompatibility of the materials were also investigated. FTIR, XPS and amino quantification demonstrated that PQQ was successfully grafted to the surface of the material. SEM and water contact angle detection proved that the surface of the material has porous mesh structure and high hydrophilicity. Blood tests showed that the functional layer reduced the number of platelet adhesion and activation degree, showing good anticoagulant ability, and the best effect was when the PQQ concentration was 300 nmol/mL. Static cell culture results showed that the functional layer improved the activity of endothelial cells and myocardial cells, and promoted the proliferation and migration of the two kinds of cells. Oxidative damage experiment showed that the functional layer could reduce the damage of H₂O₂ on endothelial cells and myocardial cells, and the best protective effect was achieved when the concentration of PQQ was 300 nmol/mL. In summary, the functional layer of PAMAM-PQQ was successfully constructed on the Titanium surface, which has good blood compatibility, cell compatibility and antioxidant damage ability, and is expected to be used for the development and application of surface modification of cardiac implantable devices.