Abstract: Flexible tactile sensors have broad application potential in electronic skin, intelligent robots, wearable electronic devices, and medical health. To solve the problems of low sensitivity and poor response/recovery performance of piezoresistive flexible tactile sensor, a near-field electrohydrodynamic direct-writing method was proposed to fabricate flexible tactile sensor based on cellulose acetate (CA)/MXene multilayer nano sheet composite fiber thin film, in which MXene nano sheets were assembled into a continuous three-dimensional (3D) conductive network with porous structure using cellulose acetate fiber with porous structure as a bridge agent. Compared with the traditional fabrication method of flexible tactile sensor, this method effectively improved the electrical performance of CA/MXene composite fiber thin film through the action of high-voltage electrostatic field, which improved the sensing performance of flexible tactile sensor. The test results show that the flexible tactile pressure sensing range of the flexible tactile sensor is 9 Pa-10.2 kPa. Within the pressure range of 9 Pa-5.6 kPa, the sensitivity of the sensor is 17.36 kPa⁻¹, and it has fast response/recovery performance (60.31/74.35 ms). The experimental results show that the fabricated flexible tactile sensor can recognize the changes of finger motion state, respiration state, and pulse signal, which has broad application prospects in human motion detection and physiological signal monitoring.