Abstract: Tension-tension fatigue tests at stress ratio R_s=0.1 were carried out on Austenitic stainless steel (316L)-Martensitic stainless steel (2Cr13) (316L-2Cr13) multilayer steel, all 316L multilayer steel and all 2Cr13 multilayer steel samples using up-and-down method and group method. The stress-life (S-N) curve was obtained and the fracture surface was analyzed. The results show that the S-N curve of multilayer steel has obvious horizontal section and definite fatigue limit due to the non-uniform microstructure in rolling state. The fatigue property of 316L-2Cr13 multilayer stainless steel composite plate is obviously better than that of all 316L or all 2Cr13 multilayer steel. When the stress ratio is 0.1, its fatigue strength can reach 286 MPa. The 316L-2Cr13 multlayer stainless steel composite plate combines the advantages of its constituent materials. 2Cr13 provides high strength to prevent rapid crack initiation of the sample, and 316L provides excellent plasticity to prevent crack propagation. The fatigue fracture surface of multilayer steel consists of fatigue source zone, crack propagation zone and final fracture zone, and the cracks nucleate at the stress concentration. In the crack propagation zone, a large number of fatigue striations exist in the 316L layer, and then dimples gradually form in the fatigue striations. At the same time, brittle transgranular fracture was observed in 2Cr13 layer, which was mainly composed of large cleavage surfaces in the later stage of crack growth. In the transient fracture zone of 316L-2Cr13 multilayer stainless steel composite plate, 2Cr13 layer presents a large number of cleavage surfaces, 316L is composed of a large number of dimples, and the layers are connected by shear dimples.