Abstract: In order to reduce the harm caused by ultraviolet rays to human beings and the damage to facilities, the cellulose product obtained from peracetic acid and maleic acid pretreatment (PAM) corn stover was used as raw material, and the corn stover-based cellulose acetate film with UV shielding function was prepared by phase inversion method using trifluoroacetic acid as catalyst and solvent in this study. With the increase of PAM pretreatment temperature, the degree of acetylation and the yield of cellulose acetate increase to 42.19% and 93.97%, respectively. The optical properties of the prepared cellulose acetate films (ZCA-80-ZCA-120) show that the corn stover-based cellulose acetate films have good UV shielding performance, UVB and UVA shielding rates of ZCA-110 reach 96.51% and 73.72%, respectively. At the same time, the relationship between the UV (200-400 nm) shielding rate, the transmittance at 660 nm (T₆₆₀) of ZCA-80-ZCA-120 and lignin content was revealed, indicating that the lignin content is the key factor determining the UV shielding performance of cellulose acetate film. A comparative study of corn stover-based cellulose acetate films without chitosan (ZCA-110) and with chitosan (CS/ZCA-5) was carried out by UV spectrophotometer, contact angle tester, XRD and FTIR, it is found that the light transmittance of the film added with chitosan is improved, the hydrophilicity is enhanced, the crystallinity is reduced. The lignocellulose-based cellulose acetate film prepared in this study will provide new ideas for the development of biodegradable UV shielding materials.