Abstract: In order to design a new type of wound dressing that does not damage the wound, has good antibacterial effect, and promotes wound healing, polyvinyl alcohol (PVA), carboxymethyl chitosan (CMCS) and sodium alginate (SA) were selected as raw materials. Silver was compounded into the PVA-CMCS-SA hydrogel, and the silver-loaded PVA-CMCS-SA hydrogel wound dressing was constructed based on 3D printing. The micro-morphology, mechanical properties, water absorption and moisture retention, biocompatibility, antibacterial properties and in vitro coagulation properties of hydrogel wound dressings were then studied. The results show that the hydrogel wound dressing prepared by 3D printing has good dimensional structural stability, good mechanical properties, and the tensile strength can reach about 1000 kPa, and both the cyclic stretching and cyclic compression properties are good. Because of the 3D printed grid structure, the 3D printed hydrogel wound dressing has good water absorption, moisture retention, in vitro clotting, air permeability and antibacterial properties. The highest antibacterial rate against Escherichia coli and Staphylococcus aureus can reach 64% and 54%. It has low cytotoxicity and good biocompatibility. The 3D printing method can combine the hydrogel with the band-aid, ensuring the practicability of the wound dressing. Studies have shown that 3D printed silver-loaded hydrogel PVA-CMCS-SA can be used as a good wound dressing material.