Abstract: In order to investigate the crack resistance of prestressed carbon fiber reinforced polymer (CFRP) reinforced steel concrete eccentrically tensioned members, eleven specimens were fabricated with different parameters such as eccentricity, level of prestress, type of prestressed tendons and vertical tension were tested under low cyclic reversed loading. The results show that the crack resistance and crack control ability of prestressed steel reinforced concrete (SRC) biased tensioning members are significantly improved compared with the ordinary SRC biased tensioning members, and the cracking load is increased by about 1.5 times. The crack resistance of prestressed SRC biased tensioning members is positively related to the prestress level, and negatively related to the eccentricity. The type of prestressed tendons has little effect on the cracking load. In addition, the 7 mm diameter prestressed CFRP tendons are stronger than the 15 mm diameter prestressed fine rolled threaded rods in controlling the number of cracks in the members, and the crack resistance of the specimens can be improved by increasing the vertical tensile force appropriately. According to the existing theory and test results, the calculation formula of cracking load is derived, and the calculated results are in good agreement with the test values.