Abstract: The self-healing oxidized sodium alginate-carboxymethyl chitosan hydrogel (OSA-CMCS) was synthesized based on dynamic imine bonds. OSA was synthesized by oxidizing the uronic acid of sodium alginate, and self-healing OSA-CMCS hydrogels with different crosslinking degrees were prepared by Schiff base reaction with carboxymethyl chitosan, analyzed the microscopic morphology, viscoelastic properties, swelling properties, self-healing properties, enzymatic degradation properties and in vitro drug release properties of OSA-CMCS hydrogels. The results show that as the ratio of OSA to CMCS increase, the degree of crosslinking of the hydrogel gradually increase and the swelling ratio gradually decrease. OSA-CMCS hydrogel has the structural characteristics of highly porous and interconnected pores, and the pore size is in the range of 20-100 μm. At room temperature, OSA-CMCS hydrogel can realize self-healing within 6 hours without external stimulation. OSA-CMCS hydrogel is degradable, and as the degree of crosslinking increase, the degradation rate slow down. OSA-CMCS hydrogel has a slow-release effect on the water-soluble drug gemcitabine, and the drug release time can reach 4 days.