Abstract: A polyacrylamide-phytic acid-polydopamine conductive molecularly imprinted membrane (PAAM-PA-PDA MIP) chemically modified electrode was prepared, characterized and applied to the quantitative detection of food additive Ponceau 4R (P4R). Based on the in-situ electrochemical polymerization and alkaline elution, a PAAM-PA-PDA MIP chemically modified electrode with molecular recognition was prepared by the modification of surface of a glassy carbon electrode (GCE) with PAAM-PA-PDA MIP. In addition, as-prepared PAAM-PA-PDA MIP chemically modified electrode was characterized by SEM, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). It is found that the PAAM-PA-PDA MIP chemically modified electrode could be applied in the electrochemical detection of P4R with linear detection range from 10 μmol/L to 200 μmol/L, high sensitivity of 0.085 A/mol/L and low detection limit of 23.6 nmol/L, which presents potential application in the practical detection of P4R.