Abstract: The graphene and metal nano-materials are excellent conductive nanomaterials. In order to construct an electrochemical sensing interface with high-efficiency active surface area, glassy carbon electrode was used as a conductive substrate, and Au-Pt nano particles/reduced graphene oxide-cellulose microfiber (Au-Pt NPs/RGO-CMF) composites were successfully prepared by drip coating combined with one-step electrodeposition. The SEM, atomic force microscopy (AFM), EDS and Raman spectroscopy analysis show that Au-Pt nanoparticles are uniformly distributed on the thin layer of RGO-CMF, and at the same time, graphene oxide (GO) reduce to RGO. Using potassium ferricyanide as a redox probe to study the electrochemical properties of the interface, under optimized experimental conditions (cyclic voltammetry electrodeposition: Potential is −1.2-0 V, period is 20, electrolyte pH value is 6, drops coated GO-CMF volume is 8 μL), the high-efficiency active surface area of Au-Pt NPs/RGO-CMF composites (3.54 cm²) is much better than that of bare glassy carbon electrode (1.52 cm²). It shows that the constructed interface has high electrocatalytic activity, which provides theoretical support for the further application of the sensor.