Abstract: The nano sodium alginate/konjac glucomannan-inulin (SA/KGM-INU) fiber membranes were prepared by electrospinning. The rheological properties of SA/KGM-INU sol were investigated by rheological characteristics analysis with different amount of SA, the structure and properties of the nano SA/KGM-INU fiber membranes were investigated by SEM, DSC, FTIR and TGA. The results show that the surface roughness of the nano fiber membranes decreases with the content of SA increasing, the viscosity increases with the content of SA increasing, and the thermal stability increases with the content of SA increasing. The preparation of nano SA/KGM-INU fiber membranes have certain guiding significance, providing experimental data and theoretical basis for further research and development of other natural polymer composites.