Biosynthesis and properties of AgNPs/chitosan composite antibacterial agent

WU Chengzong; WEI Ya'nan; HUANG Ting; MIAO Yuying; ZHANG Yidan; LIU Yuyang; LIU Xiaoli; ZHAO Yancui; QIAO Hongjin; WANG Lei

doi:10.13801/j.cnki.fhclxb.20230117.003

Volume 40 Issue 10

Oct. 2023

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WU Chengzong, WEI Ya'nan, HUANG Ting, et al. Biosynthesis and properties of AgNPs/chitosan composite antibacterial agent[J]. Acta Materiae Compositae Sinica, 2023, 40(10): 5871-5883. doi: 10.13801/j.cnki.fhclxb.20230117.003

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WU Chengzong, WEI Ya'nan, HUANG Ting, et al. Biosynthesis and properties of AgNPs/chitosan composite antibacterial agent[J]. Acta Materiae Compositae Sinica, 2023, 40(10): 5871-5883. doi: 10.13801/j.cnki.fhclxb.20230117.003

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Biosynthesis and properties of AgNPs/chitosan composite antibacterial agent

doi: 10.13801/j.cnki.fhclxb.20230117.003

College of Life Sciences, Ludong University, Yantai 264025, China

Funds: Shandong Natural Science Foundation Project (ZR2021MC102); Science and Technology Development Plan of Yantai (2022XDRH010)

Received Date: 2022-11-14
Accepted Date: 2023-01-08
Rev Recd Date: 2022-12-12

Available Online: 2023-01-18

Publish Date: 2023-10-15

Abstract

Abstract

In order to prepare a new type of safe, stable and efficient nano silver composite antibacterial agent, silver nanoparticles/chitosan (AgNPs/CS) was biosynthesized by one-step method using Chimonanthus praeco petal extract and chitosan as reducing agent and stabilizing agent, respectively. The optimal preparation procedure was determined by single factor experiment. The products were characterized by UV-vis absorption spectroscopy, TEM and X-ray diffraction, and their antibacterial activity, anti-drug resistance, stability as well as biological safety were comprehensively evaluated. The experimental results show that the AgNPs/CS has the characteristic absorption peak of AgNPs at 451 nm, and the evenly dispersed AgNPs are spherical with average diameter of 12.83 nm, and the crystals have a face-centered cubic structure. The AgNPs/CS show antibacterial activity against aquatic pathogens both in vitro and in vivo, and has excellent anti-drug resistance, biosafety and stability. Therefore, AgNPs/CS is an ideal composite antibacterial agent, which has a prospective application in aquaculture field.
- silver nanoparticles,
- chitosan,
- biosynthesis,
- antibacterial activity,
- drug resistance,
- stability
Long Abstrsct
Innovation Points

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References(36)

References

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