Preparation of conductive molecularly imprinted membrane chemically modified electrode and electrochemical detection of Ponceau 4R
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摘要: 报道了一种聚丙烯酰胺(PAAM)-植酸(PA)-聚多巴胺(PDA)导电分子印迹膜(PAAM-PA-PDA MIP)化学修饰电极的制备、表征及其在电化学定量检测食品添加剂胭脂红(P4R)中的应用。即通过原位电聚合和碱液洗脱的方法在玻碳电极(GCE)表面制得具有分子识别作用的导电分子印迹膜(PAAM-PA-PDA MIP)化学修饰电极，并利用SEM、循环伏安法(CV)及交流阻抗法(EIS)对该导电分子印迹膜化学修饰电极的表面形貌和电化学性能进行表征。研究结果表明该方法所制备的导电分子印迹膜化学修饰电极具有良好的电化学检测性能和应用前景，其对P4R的线性检测区间为10~200 μmol/L，灵敏度为0.085 A/mol/L，检测限可达23.6 nmol/L，并可有效地应用于P4R实际样品的分析检测。Abstract: A polyacrylamide-phytic acid-polydopamine conductive molecularly imprinted membrane (PAAM-PA-PDA MIP) chemically modified electrode was prepared, characterized and applied to the quantitative detection of food additive Ponceau 4R (P4R). Based on the in-situ electrochemical polymerization and alkaline elution, a PAAM-PA-PDA MIP chemically modified electrode with molecular recognition was prepared by the modification of surface of a glassy carbon electrode (GCE) with PAAM-PA-PDA MIP. In addition, as-prepared PAAM-PA-PDA MIP chemically modified electrode was characterized by SEM, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). It is found that the PAAM-PA-PDA MIP chemically modified electrode could be applied in the electrochemical detection of P4R with linear detection range from 10 μmol/L to 200 μmol/L, high sensitivity of 0.085 A/mol/L and low detection limit of 23.6 nmol/L, which presents potential application in the practical detection of P4R.
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图 1 原位聚合聚丙烯酰胺(PAAM) (a)、原位聚合PAAM-植酸(PA)-聚多巴胺(PDA)导电分子印迹膜(MIP)的循环伏安图 (b)，PAAM-PA-PDA MIP原位电聚合结合碱液洗脱的示意图 (c)
Figure 1. In-situ electrochemical polymerization of polyacrylamide (PAAM) (a), in-situ electrochemical polymerization of PAAM-phytic acid (PA)-polydopamine (PDA) conductive molecularly imprinted membrane (MIP) (b) by CV curves; schematic illustration of PAAM-PA-PDA MIP using the in-situ electrochemical polymerization and alkaline elution (c)
GCE—Glassy carbon electrode; PA—Polyamide; DA—Dopareine; AAM—Acrylamide; P4R—Ponceau 4R
图 2 PAAM ((a)、(c)) 和PAAM-PA-PDA MIP ((b)、(d)) 的SEM图像
Figure 2. SEM images of PAAM ((a), (c)) and PAAM-PA-PDA MIP ((b), (d))
图 3 [Fe(CN)6]3−探针离子在PAAM修饰电极、PAAM-PA-PDAMIP修饰电极和非分子印迹膜(NIP)修饰电极上的循环伏安曲线(插图为PAAM修饰电极的循环伏安曲线)
Figure 3. CV curves obtained for the [Fe(CN)6]3− as electrochemical probe ion on the PAAM modified electrode, PAAM-PA-PDA MIP modified electrode and non-molecularly imprinted (NIP) modified electrode (Inset is the CV curve of PAAM modified electrode)
图 4 P4R(20 μmol/L)在PAAM-PA-PDA MIP修饰电极和NIP修饰电极上的CV曲线
Figure 4. CV curves of 20 μmol/L P4R at the PAAM-PA-PDA MIP modified electrode and NIP modified electrode
图 5 不同浓度下(10~300 μmol/L)P4R在PAAM-PA-PDA MIP修饰电极上的DPV曲线(插图为DPV峰电流与P4R浓度之间的标准曲线)
Figure 5. DPV curves obtained for the different P4R concentrations (10-300 μmol/L) at the PAAM-PA-PDA MIP modified electrode (Inset is the standard curve based on the relationship between peak current and P4R concentration)
图 6 PAAM-PA-PDA MIP修饰电极应用于实际果汁样品检测所得到的DPV曲线
Figure 6. DPV curve obtained for a juice sample containing P4R by using the PAAM-PA-PDA MIP modified electrode
图 7 PAAM-PA-PDA MIP修饰电极应用于抗干扰实验所得到的I-T曲线
Figure 7. I-T curve obtained for different interfering component at the PAAM-PA-PDA MIP modified electrode
UA—Uric acid; Glu—Glucose; AA—Ascorbic acid
表 1 与其他检测P4R的文献报道进行性能对比
Table 1. Comparison of the proposed electrode with other electrodes for the determination of P4R
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