Preparation and properties of nano-SiO2/anthocyanidin/ regenerated cellulose smart colorimetric sensing film for food freshness monitoring
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摘要: 智能食品包装能够在实时质量监测方面弥补传统包装的不足，开发一种低成本、对人类健康无害和绿色环保的食品新鲜度智能指示标签，对食品安全有重要意义。本研究基于再生纤维素(RC)膜，利用从紫甘蓝中提取的花青素和纳米SiO2，制备了一种可生物降解的智能比色传感膜(RCG-PCE-Si)。该膜具有良好的力学性能(承受最大应力为67.1 MPa，最大拉伸应变为45.50 %)和超疏水性(水接触角为152.8°)，且对紫外线有一定的阻隔能力，可用于生鲜类食品新鲜度的实时监测。通过测定总挥发性盐基氮(TVBN)和pH值，验证了该膜应用于虾新鲜度监测的有效性，其颜色明显的由品红色变为水红色，再变为紫灰色，最后变为灰绿色，有效地指示了虾腐败。本研究所开发的比色传感膜将有望作为具有良好力学性能和超疏水性的智能比色标签，并且应用于可对食品进行实时新鲜度监测的智能包装中。Abstract: Smart food packaging can make up for the shortcomings of traditional packaging in real-time quality monitoring, so exploiting a low cost, harmless to human health and green food freshness intelligent indicator label is of great significance for food safety. Therefore, in this study, we employed regenerated cellulose (RC) films, anthocyanidin extracted from red cabbage and nano-SiO2 to fabricate a biodegradable smart colorimetric sensing film (RCG-PCE-Si). This film has good mechanical properties (maximum stress is 67.1 MPa, and maximum tensile strain is 45.50%) and super-hydrophobicity (water contact angle is 152.8°), and has a certain barrier ability to ultraviolet light, which can be used for real-time monitoring of fresh food freshness. In addition, the effectiveness of the film in detecting the freshness of shrimp was confirmed by measuring the total volatile basic nitrogen (TVBN) and pH value, and its color obviously changes from magenta to water-red, then to grey-purple, and then to grey-green, effectively indicating the spoilage of shrimp. The colorimetric sensing film developed here would be used as an intelligent colorimetric label with good mechanical properties and super-hydrophobicity, and can be applied to intelligent packaging that can monitor the freshness of food in real time.
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图 2 不同pH下PCE溶液照片(a)和紫外-可见光谱(b)
Figure 2. Photograph(a) and UV-vis spectra(b) of PCE solution at different pH values
图 3 不同薄膜(a)、不同pH下PCE溶液(b)和RC、PCE溶液以及SiO2(c)的FTIR图谱
Figure 3. FTIR spectra of films(a), PCE solution at different pH values(b) and RC, PCE, SiO2(c)
图 6 不同薄膜的拉伸应力-应变曲线(a)、抗拉强度图(b)、断裂伸长率图(c)和厚度散点图(d)
Figure 6. Stress-strain(a), tensile strength(b), elongation at break(c) and thickness(d) of films
图 11 比色传感膜对不同pH溶液(pH = 5.0,7.0,10.0,12.0)的超疏水性图片
Figure 11. Images of RCG-PCE-Si superhydrophobic performance to different pH aqueous solutions (pH = 5.0,7.0,10.0,12.0)
图 15 不同温度条件和不同贮存天数下比色传感膜的颜色(a)、色差值(b)、虾的TVBN含量(c)和虾的pH值(d)
Figure 15. the color(a) and ΔE(b) of RCG-PCE-Si, TVBN content(c) and pH value(d) of shrimp under different temperature conditions for different storage days
表 1 不同膜样品的命名
Table 1. Naming of films
Sample name Glycerol/wt% Soak in PCE Spray SiO2 RC 0 − − RCG 10 − − RC-PCE 0 + − RCG-PCE 10 + − RC-PCE-Si 0 + + RCG-PCE-Si 10 + + Notes: RC—regenerated cellulose; PCE—purple cabbage extract; “+” means this sample performed this step; “−” means this sample had not perform this step. 
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