Preparation and properties of modified soy protein-bacterial cellulose composites for air filtration
-
摘要: 采用大豆蛋白和细菌纤维素(BC)为原材料,制备环保型空气过滤复合材料,用于过滤污染空气。首先,采用Nagano法对大豆蛋白进行提纯和成分分离,得到溶解度较高的7S和11S成分。然后,用丙烯酸对大豆分离蛋白(SPI)、7S和11S进行改性处理,使埋藏于大豆蛋白内部的官能团充分暴露。最后,将处理后的大豆蛋白与BC复合,制备出改性大豆蛋白-BC (MSPI-BC、M7S-BC和M11S-BC)复合材料。评估了改性大豆蛋白-BC复合材料的微观形貌及其对污染空气的过滤效率、吸附性能和透气性等。结果表明,MSPI在BC中分布极为不均匀,在多处产生聚集;而M7S和M11S蛋白均匀包覆在BC表面,无聚集现象。MSPI-BC、M7S-BC和M11S-BC复合材料对PM2.5的过滤效率分别为73.07%±0.02%、82.13%±0.01%和85.44%±0.02%。与MSPI-BC复合材料相比,M7S-BC和M11S-BC复合材料对空气中颗粒污染物的吸附量更大。本文制备出的改性大豆蛋白-BC复合材料结构稳定,具有较高的过滤效率,且环保无污染,在空气过滤领域有广阔的应用价值。Abstract: Environmentally-friendly air filtration composite materials were prepared to filter polluted air using soybean protein and bacterial cellulose (BC) as raw materials. 7S and 11S with high solubility were firstly obtained through purification and separation of soy protein by adopting Nagano. Thereafter, soy protein isolate (SPI), 7S and 11S were treated with acrylic acid to unfold the polypeptide chains. Finally, modified soy protein-BC (MSPI-BC, M7S-BC and M11S-BC) composites were prepared by adding soy protein treated with acrylic acid to BC. Besides, the morphology, air filtration efficiency, adsorption capacity and air penetration of the modified soy protein-BC composites were evaluated. The results show that MSPI unevenly distributes in BC, aggregating on the surface of BC fibers. While M7S and M11S uniformly cover on the surface of BC fibers, without aggregation. The air filtration efficiency of MSPI-BC, M7S-BC and M11S-BC composites for particulate pollutants is 73.07%±0.02%, 82.13%±0.01% and 85.44%±0.02%, respectively. Moreover, M7S-BC and M11S-BC composites adsorb more particulate pollutants than MSPI-BC composite. The prepared modified soy protein-BC composites in this study own stable structure and high air filtration efficiency, and they are environmentally-friendly, thus being potential to be used in the field of air filtration.
-
Key words:
- soy protein /
- bacterial cellulose /
- acrylic acid /
- air filtration /
- adsorption capability
-
表 1 改性大豆蛋白-BC复合材料配比
Table 1. Proportion of modified soy protein-BC composites
Sample Soy protein/wt% BC/wt% MSPI-BC 7.41 92.59 M7S-BC 44.44 55.56 M11S-BC 44.44 55.56 表 2 丙烯酸改性大豆蛋白-BC复合材料的空气过滤效率和透气性
Table 2. Air filtration efficiency and penetration rate of acrylic acid modified soy protein-BC composites
Sample Air filtration efficiency/% Penetration rate/% Areal density/
(g·m−2)MSPI-BC 73.07±0.02 65.42±0.30 103±5 M7S-BC 82.13±0.01 64.72±0.30 105±5 M11S-BC 85.44±0.02 67.50±0.40 98±4 -
[1] LIU C, HSU P C, LEE H W, et al. Transparent air filter for high-efficiency PM2.5 capture[J]. Nature Communications,2015,6:6205. doi: 10.1038/ncomms7205 [2] MIASKIEWICZ-PESKA E, LEBKOWSKA M. Comparison of aerosol and bioaerosol collection on air filters[J]. Aerobiologia,2012,28(2):185-193. doi: 10.1007/s10453-011-9223-1 [3] 石小丽, 潘倩倩. 复合高效空气过滤非织造材料的制备及其性能[J]. 实验室研究与探索, 2011, 30(9):35-38. doi: 10.3969/j.issn.1006-7167.2011.09.011SHI X L, PAN Q Q. Preparation and properties of tier composite nonwovens for high efficiency air filtration[J]. Research and Exploration in Laboratory,2011,30(9):35-38(in Chinese). doi: 10.3969/j.issn.1006-7167.2011.09.011 [4] LUBASOVA D, NETRAVALI A, PARKER J, et al. Bacterial filtration efficiency of green soy protein based nanofiber air filter[J]. Journal of Nanoscience & Nanotechnology,2014,14(7):4891-4898. [5] PENG I C, QUASS D W, DAYTON W R, et al. The physicochemical and functional properties of soybean 11S globulin: A review[J]. Cereal Chemistry,1984,61(6):480-490. [6] 周瑞宝, 周兵. 大豆7S和11S球蛋白的结构和功能性质[J]. 中国粮油学报, 1998, 13(6):39-42. doi: 10.3321/j.issn:1003-0174.1998.06.011ZHOU R B, ZHOU B. The structure and functional properties of soybean 7S and 11S globulin proteins[J]. Journal of the Chinese Cereals and Oils Association,1998,13(6):39-42(in Chinese). doi: 10.3321/j.issn:1003-0174.1998.06.011 [7] SAMOTO M, MAEBUCHI M, MIYAZAKI C, et al. Abundant proteins associated with lecithin in soy protein isolate[J]. Food Chemistry,2007,102(1):317-322. doi: 10.1016/j.foodchem.2006.05.054 [8] SIRISON J, MATSUMIYA K, SAMOTO M, et al. Solubility of soy lipophilic proteins: Comparison with other soy protein fractions[J]. Bioscience Biotechnology & Biochemistry,2017,81(4):790-802. [9] KUMAR R, CHOUDHARY V, MISHRA S, et al. Adhesives and plastics based on soy protein products[J]. Industrial Crops & Products,2002,16(3):155-172. [10] SALAS C, AGO M, LUCIA L A, et al. Synthesis of soy protein-lignin nanofibers by solution electrospinning[J]. Reactive & Functional Polymers,2014,85:221-227. [11] ZHAO S, YAO J, FEI X, et al. An antimicrobial film by embedding in situ synthesized silver nanoparticles in soy protein isolate[J]. Materials Letters,2013,95(3):142-144. [12] SANTOS T C, HORING B, REISE K, et al. In vivo performance of chitosan/soy-based membranes as wound dressing devices for acute skin wounds[J]. Tissue Engineering Part A,2013,19(7-8):860-869. doi: 10.1089/ten.tea.2011.0651 [13] 刘杰, 周浩, 黄郁芳, 等. 聚乙二醇化学改性的大豆分离蛋白凝胶[J]. 高等学校化学学报, 2018, 39(2):390-396. doi: 10.7503/cjcu20170449LIU J, ZHOU H, HUANG Y F, et al. Polyethylene glycol chemically modified soy protein isolate hydrogel[J]. Chemical Journal of Chinese Universities,2018,39(2):390-396(in Chinese). doi: 10.7503/cjcu20170449 [14] CHENG X, ZENG X, LI D, et al. TPGS-grafted and acid-responsive soy protein nanogels for efficient intracellular drug release, accumulation, penetration in 3D tumor spheroids of drug-resistant cancer cells[J]. Materials Science & Engineering C: Materials for Biological Applications,2019,102:863-875. [15] ZHAO Y, HE M, ZHAO L, et al. Epichlorohydrin-cross-linked hydroxyethyl cellulose/soy protein isolate composite films as biocompatible and biodegradable implants for tissue engineering[J]. ACS Applied Materials & Interfaces,2016,8(4):2781-2795. [16] SOUZANDEH H, JOHNSON K S, WANG Y, et al. Soy-protein-based nanofabrics for highly efficient and multifunctional air filtration[J]. ACS Applied Materials & Interfaces,2016,8(31):20023-20031. [17] SHAH N, UI-ISLAM M, KHATTAK W A, et al. Overview of bacterial cellulose composites: A multipurpose advanced material[J]. Carbohydrate Polymers,2013,98(2):1585-1598. doi: 10.1016/j.carbpol.2013.08.018 [18] LIU X, SOUZANDEH H, ZHENG Y, et al. Soy protein isolate/bacterial cellulose composite membranes for high efficiency particulate air filtration[J]. Composites Science and Technology,2017,138:124-133. doi: 10.1016/j.compscitech.2016.11.022 [19] NAGANO T, HIROTSUKA M, MORI H, et al. Dynamic viscoelastic study on the gelation of 7 S globulin from soybeans[J]. Journal of Agricultural & Food Chemistry,1992,40(6):941-944. [20] LIN T C, KRISHNASWAMY G, CHI D S. Incense smoke: Clinical, structural and molecular effects on airway disease[J]. Clinical and Molecular Allergy,2008,6(1):3. doi: 10.1186/1476-7961-6-3 [21] TIAN H, XU G, YANG B, et al. Microstructure and mechanical properties of soy protein/agar blend films: Effect of composition and processing methods[J]. Journal of Food Engineering,2011,107(1):21-26. doi: 10.1016/j.jfoodeng.2011.06.008 [22] WANG Z, ZHOU J, WANG X X, et al. The effects of ultrasonic/microwave assisted treatment on the water vapor barrier properties of soybean protein isolate-based oleic acid/stearic acid blend edible films[J]. Food Hydrocolloids,2014,35:51-58. doi: 10.1016/j.foodhyd.2013.07.006 [23] 程宝箴, 范志婕, 张海丽. 丙烯酸改性废革屑胶原蛋白的研究[J]. 皮革科学与工程, 2014, 24(5):10-15.CHENG B Z, FAN Z J, ZHANG H L. Research of the collagen modified from leather waste by acrylic acids[J]. Leather Science and Engineering,2014,24(5):10-15(in Chinese). [24] CHO D, NAYDICH A, FREY M W, et al. Further improvement of air filtration efficiency of cellulose filters coated with nanofibers via inclusion of electrostatically active nanoparticles[J]. Polymer,2013,54(9):2364-2372. doi: 10.1016/j.polymer.2013.02.034 [25] 顾振宇, 江美都, 付道才, 等. 大豆分离蛋白乳化性的研究[J]. 中国粮油学报, 2000, 15(2):32-35. doi: 10.3321/j.issn:1003-0174.2000.02.008GU Z Y, JIANG M D, FU D C, et al. Study on emulsifying properties of soy protein isolate[J]. Journal of the Chinese Cereals and Oils Association,2000,15(2):32-35(in Chinese). doi: 10.3321/j.issn:1003-0174.2000.02.008 [26] JOHNSON T J, OLFERT J S, CABOT R, et al. Transient measurement of the effective particle density of cigarette smoke[J]. Journal of Aerosol Science,2015,87:63-74. doi: 10.1016/j.jaerosci.2015.05.006 [27] WRIGHT C. Standardized methods for the regulation of cigarette-smoke constituents[J]. Trends in Analytical Chemistry,2015,66:118-127. doi: 10.1016/j.trac.2014.11.011