纳米核壳型Ag@Fe<sub>3</sub>O<sub>4</sub>复合材料的制备、催化及抑菌性能

郭少波; 梁艳莉; 季晓晖; 兰阿峰; 黄佩; 杜全超; 马剑琪

doi:10.13801/j.cnki.fhclxb.20200623.001

纳米核壳型Ag@Fe₃O₄复合材料的制备、催化及抑菌性能

doi: 10.13801/j.cnki.fhclxb.20200623.001

1.
陕西理工大学　化学与环境科学学院，汉中 723000
2.
陕西理工大学　生物科学与工程学院，汉中 723000

基金项目: 陕西省自然科学基础研究计划 (2020JM-602)

详细信息

通讯作者:
马剑琪，博士，教授，硕士生导师，研究方向为贵金属催化　E-mail：jiqnqima@163.com

中图分类号: O649
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出版历程
- 收稿日期: 2020-04-30
- 录用日期: 2020-06-05
- 网络出版日期: 2020-06-23
- 刊出日期: 2021-03-15

Preparation, catalytic property and antibacterial property of Ag@Fe₃O₄ core-shell composite nanomaterials

1.
School of Chemistry and Environmental Science, Shaanxi University of Technology, Hanzhong 723000, China
2.
School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China

摘要

摘要: 采用“一锅法”制备纳米核-壳结构的Ag@Fe₃O₄复合材料。利用TEM、XRD、UV-vis DRS、振动探针式磁强计(VSM)对Ag@Fe₃O₄复合材料进行表征。以甲基橙为目标污染物，研究Ag@Fe₃O₄复合材料在过量NaBH₄介质中加氢还原的催化活性，并探讨其催化机制；以单质Ag和Fe₃O₄作参比，研究Ag@Fe₃O₄复合材料对金黄色葡萄球菌和大肠杆菌的抑菌性能。结果表明，在10 min内，Ag@Fe₃O₄复合材料对甲基橙的加氢还原催化率为98%以上，且活性Ag转移电子至甲基橙的N=N键，使其断裂还原成对氨基苯磺酸钠和对二氨基苯；抑菌实验表明，Ag@Fe₃O₄复合材料比单质Ag具有更强的抑菌活性，并对细胞壁中含有更薄的磷脂双分子层的大肠杆菌更为敏感。
- 复合材料 /
- Ag /
- 催化 /
- 抑菌 /
- 核壳型 /
- Fe₃O₄
Abstract: The Ag@Fe₃O₄ composites with nano core-shell structure were successfully prepared by a one-pot method. The resulting Ag@Fe₃O₄ composites were characterized by TEM, XRD, UV-vis DRS and vibrating sample magnetometry (VSM). The catalytic performance and mechanism of Ag@Fe₃O₄ composites were investigated by photometrically monitoring the reduction of methyl orange in the presence of excess of NaBH₄. Furthermore, the antibacterial of Ag@Fe₃O₄ composites against Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) was studied by the paper diffusion experiment using Ag and Fe₃O₄ as the references. The results indicate that over 98% of methyl orange is catalytically degraded within 10 min. This superior catalytic activity may be resulted from transferring electron to N=N bond by Ag, thereby causing N=N fracture and generating sodium 4-aminobenzenesulfonate and p-diaminobenzene. Antibacterial experiment shows that Ag@Fe₃O₄ composites more excellent bacteriostatic activity than Ag and is more sensitive to E.coli than to S.aureus. The main reason can be ascribed to the fact that phospholipid bilayer in cell wall of E.coli is thinner than that of S.aureus.
- composite materials /
- Ag /
- catalytic /
- inhibition /
- the Core-shell type /
- Fe₃O₄

HTML全文

图 1 Ag@Fe₃O₄复合材料((a), (b))、Ag纳米颗粒(SNP) ((c), (d))和Fe₃O₄ ((e), (f))的TEM图像

Figure 1. TEM images of Ag@Fe₃O₄ composite ((a), (b)), Ag nanoparticle (SNP) ((c), (d)) and Fe₃O₄ ((e), (f))

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图 2 Fe₃O₄、Ag和Ag@ Fe₃O₄复合材料的XRD图谱

Figure 2. XRD patterns of Fe₃O₄, Ag and Ag@Fe₃O₄ composite

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图 3 Fe₃O₄、Ag和Ag@Fe₃O₄复合材料的UV-Vis漫反射吸收图谱

Figure 3. UV-Vis diffuse reflectance spectra of Fe₃O₄, Ag and Ag@Fe₃O₄ composite

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图 4 Fe₃O₄和Ag@Fe₃O₄复合材料的饱和磁强度

Figure 4. Saturation magnetization of Fe₃O₄ and Ag@Fe₃O₄ composite

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图 5 相同质量(0.1 mg)的Fe₃O₄ (a)、SNP (b)和Ag@Fe₃O₄复合材料(c)对甲基橙催化降解过程吸光度的变化及标准品对氨基苯磺酸钠的吸收光谱(d)

Figure 5. Absorbance change during catalytic with same mass (0.1 mg) degradation of methyl orange using Fe₃O₄ (a), SNP (b) and Ag@Fe₃O₄ composite (c) and absorption spectra of standard sodium sulfanilate (d)

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图 6 甲基橙直接烷化的机制

Figure 6. Mechanism path for direct alkylation of methyl orang

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图 7 不同浓度的水、SNP、Fe₃O₄和Ag@Fe₃O₄复合材料对大肠杆菌(E. coli)和金黄色葡萄球菌(S. aureus)的滤纸片扩散照片

Figure 7. Inhibition zones of as-synthesized water, SNP, Fe₃O₄e and Ag@Fe₃O₄ composite with different concentrations against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus)

O—Water; A—SNP; B—Fe₃O₄; C—Ag@Fe₃O₄; a1, b1—10 µg/mL; a2, b2—20 µg/mL; a3, b3—30 µg/mL; a4, b4—40 µg/mL

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图 8 Ag@Fe₃O₄复合材料对E. coli和S.aureus的抑菌机制

Figure 8. Antibacterial mechanism of Ag@Fe₃O₄ composite to E. coli and S. aureus bacteria

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表 1 实验所用材料、剂量和不同材料对甲基橙的催化效率

Table 1. Table information of experimental material, dosage and catalytic efficiency of different materials for methyl orange

Sample	Concentration	Volume/mL	Catalytic efficiency R/%
Methyl orange	0.25 mmol/L	1	—
NaBH₄	0.02 mol/L	2	—
Fe₃O₄	0.5 mg/mL	0.2	0
Ag	0.5 mg/mL	0.2	46
Ag@Fe₃O₄	0.5 mg/mL	0.2	98
Note: R=(C₀–C)/C₀×100%, C₀—Initial concentration, C—Residual concentration.

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