Abstract: The Ag-carried tetracycline-mulberry bast carboxymethyl cellulose/polyvinyl alcohol(Ag-T-CMC/PVA)composite films were prepared by solution casting method. And the structure and properties of Ag-T-CMC/PVA composite films were investigated by XRD, SEM and DSC. The results show that with the increase of Ag-T-CMC content, the mechanical properties, water resistance and antibacterial properties of Ag-T-CMC/PVA composite films are improved. When the mass ratio of Ag-T-CMC to PVA is 2%, the mechanical properties of the Ag-T-CMC/PVA composite film are the best and the tensile strength increases by 3.4%. SEM analysis shows that Ag-T-CMC is uniformly dispersed in Ag-T-CMC/PVA composite films, showing good compatibility, and with the increasing Ag-T-CMC content, the faults gradually become smooth plane. When the mass ratio of Ag-T-CMC to PVA is 2%, the faults are the smoothest. The water absorption test shows that Ag-T-CMC can significantly reduce the water absorption of Ag-T-CMC/PVA composite films. The antibacterial test shows that the Ag-T-CMC/PVA composite films have certain antibacterial effect on Escherichia coli and Staphylococcus aureus, and with the increase of Ag-T-CMC content, the diameter of the antibacterial circle becomes larger and the antibacterial effect is enhanced.