纯钛表面磷酸氢钙-羟基磷灰石膜层的化学转化及成骨细胞反应

刘冰; 肖桂勇; 吕宇鹏

doi:10.13801/j.cnki.fhclxb.20170216.002

纯钛表面磷酸氢钙-羟基磷灰石膜层的化学转化及成骨细胞反应

doi: 10.13801/j.cnki.fhclxb.20170216.002

刘冰^1,2,
肖桂勇^1,2,
吕宇鹏^1,2,

1.
山东大学材料液固结构演变与加工教育部重点实验室, 济南 250061;
2.
山东大学材料科学与工程学院, 济南 250061

基金项目: 山东省科技发展计划（2014GGX102031）；江苏省青年基金（BK20140412）

详细信息

通讯作者:
吕宇鹏,博士,教授,博士生导师,研究方向为生物医用金属材料 E-mail:biosdu@sdu.edu.cn

中图分类号: TB333
计量
- 文章访问数: 940
- PDF下载量: 276
- 被引次数: 0
出版历程
- 收稿日期: 2016-11-22
- 修回日期: 2017-01-10
- 刊出日期: 2017-11-15

Preparation and cytocompatibility of brushite-hydroxyapatite coating on Ti by chemical conversion

LIU Bing^1,2,
XIAO Guiyong^1,2,
LV Yupeng^{1,2
,}

1.
Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, Shandong University, Ji'nan 250061, China;
2.
School of Materials Science and Engineering, Shandong University, Ji'nan 250061, China

摘要

摘要: 为了提高医用纯钛的骨修复效果，利用化学转化技术在纯钛表面制备了一层含钙磷化合物的磷酸盐（CaP）转化膜，并通过碱处理，对该转化膜进行羟基转化。利用XRD、FESEM和电化学工作站对CaP转化膜的成分、微观结构和耐蚀性能分别进行分析表征，通过MC3T3-E1小鼠成骨细胞培养，对覆膜样本的细胞相容性进行评价。研究结果表明，纯钛表面CaP转化膜的主要成分为磷酸氢钙（CaHPO₄ · 2H₂O），膜层晶体呈块状无规则多级排列，经过碱处理后，膜层晶体表面粗糙度增加，并有新的细小颗粒生成，晶体部分转化为羟基磷灰石（HA）。电化学分析结果表明，经过碱液处理的HA转化膜在生理盐水中表现出了比CaHPO₄ · 2H₂O转化膜更好的耐蚀性能。体外细胞培养试验证明，覆膜纯钛样本能够有效促进成骨细胞黏附、细胞增殖和分化。
- 纯钛 /
- 转化膜 /
- 磷酸氢钙 /
- 羟基磷灰石 /
- 成骨细胞
Abstract: In order to enhance the osseointegration of Ti implant, chemical conversion technology was used to prepare a calcium phosphate (CaP) coating on Ti. The alkali treatment was conducted to rise a secondary coating on the primary coated Ti. The phase composition, microstructure and corrosion resistance of the CaP coated Ti (Ti-CaP) samples were investigated using the XRD, field emission SEM and electrochemical measurements, respectively. The cytocompatibility was evaluated by the cell attachment, proliferation and alkaline phosphatase (ALP) activity of MC3T3-E1 cells. The results show that the primary coating formed on Ti is mainly composed of brushite (CaHPO₄·2H₂O) with a blocky structure. After alkali treatment, the brushite crystals are partly transformed to hydroxyapatite (HA) crystals with a coarser surface and some newborn crystals. The electrochemical measurements reveal that the corrosion resistance of the HA coated Ti is better than that of the brushite coated Ti. In vitro cell tests demonstrate that the surface of the coated Ti samples exhibit better cytocompatibility than that of pure Ti.
- Ti /
- conversion coating /
- brushite /
- hydroxyapatite /
- osteoblast