Preparation and application of Fmoc-Dphenylalanine/hyaluronic acid composite double-network hydrogel
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摘要:
将N-芴甲氧羰基-D-苯丙氨酸(Fmoc-DPhe)和甲基丙烯酸缩水甘油酯(Glycidyl methacrylate,GMA)修饰的透明质酸(HA-GMA)在磷酸缓冲液中共混加热,冷却后Fmoc-DPhe分子先自组装形成超分子水凝胶,超分子水凝胶中的HA-GMA再经光照引发交联制备双网络复合水凝胶。研究该双网络水凝胶的力学性能、光学性质、微观形貌、药物缓释能力和抑菌性能。研究结果表明,双网络水凝胶比HA-GMA单网络水凝胶的力学性能强一倍左右且HA-GMA网络存在于双网络水凝胶中;光学性质显示双网络水凝胶中存在Fmoc-DPhe网络;微观形貌表明有两种水凝胶网络均存在于复合水凝胶中。当复合水凝胶包裹小分子模拟药物后,复合水凝胶达到模拟药物最大累积释放量的时间要比Fmoc-DPhe单网络水凝胶的长6 h;针对革兰氏阳性细菌的抑菌能力研究显示,双网络水凝胶的抑菌效果也比Fmoc-DPhe单网络水凝胶的更好。
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关键词:
- D型Fmoc-苯丙氨酸 /
- 透明质酸 /
- 复合水凝胶 /
- 药物释放 /
- 抑菌性能
Abstract:N-fluoren-methoxycarbonyl-D-Phenylalanine (Fmoc-DPhe) and glycidyl methacrylate (GMA)-modified hyaluronic acid (HA-GMA) were mixed and heated in phosphoric acid buffer. After cooling, Fmoc-DPhe molecules were self-assemble into supermolecular hydrogel followed by forming double network hydrogel with HA-GMA under UV light irradiation. The mechanical properties, optical properties, microstructure, drug release and bacteriostatic properties of the composite hydrogel were studied. The results show that the double-network hydrogels are about twice as strong as those of HA-GMA single-network hydrogel, and the HA-GMA network exists in the double-network hydrogels. Optical properties show the existence of Fmoc-DPhe network in the double network hydrogels. The microstructure indicates that there are two kinds of hydrogel networks in the composite hydrogel. Moreover, after loading the drug, the double-network hydrogel takes 6 hours longer to reach the maximum drug accumulation rate than the Fmoc-DPhe single-network hydrogel. The antibacterial activity of the double-network hydrogel against gram-positive bacteria is also better than that of the Fmoc-DPhe single-network hydrogel.
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Key words:
- Fmoc-DPhe /
- hyaluronic acid /
- composite hydrogel /
- drug-release /
- antibacterial activity
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表 1 压缩实验测试水凝胶的压缩强度和压缩应变
Table 1. Fracture stresses and strains of hydrogels under compression
Hydrogel Fmoc-DPhe hydrogel HA-GMA hydrogel Composite hydrogel Fracture stress/kPa 6.9 0.6 36.1 Fracture strain/% 47.11 53.86 48.93 -
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